Urease Antikörper (HRP)

Produktnummer ABIN7565994

Produktdetails anti-Urease Antikörper (HRP)

Target: Urease (URE)

Reaktivität: Schwertbohne

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Urease Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB)

Verwendungszweck: Urease Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Urease [Jack Bean].

Produktmerkmale: Synonyms: rabbit anti-Urease Antibody HRP Conjugation, Peroxidase Conjugated rabbit anti-Urease Antibody, Urea Amidohydrolase antibody

Aufreinigung: Urease is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Urease [Jack Bean]

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Urease peroxidase conjugated antibody has been tested by dot blot and is suitable to be assayed against 1.0 μg of Urease [Jack Bean] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:500 to 1:2,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:50 - 1:500 Immunoprecipitation Dilution: User Optimized ELISA Dilution: 1:500 - 1:2,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Urease

Target: Urease (URE)

Andere Bezeichnung: Urease (URE Produkte)

Hintergrund: Background: Urease is an enzyme involved in nitrogen metabolism. It catalyzes the hydrolysis of urea into carbon dioxide and ammonia.

UniProt: P07374