PKM2 Antikörper (AA 255-350)

Produktnummer ABIN724910

Produktdetails anti-PKM2 Antikörper

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Bindungsspezifität: AA 255-350

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PKM2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Cow,Pig,Horse,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse Pyruvate kinase PKM

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-PKM2 Antikörper (ABIN724910)

Li, He, Zhang, Miao: "Differential proteome and gene expression reveal response to carbon ion irradiation in pubertal mice testes." in: Toxicology letters, Vol. 225, Issue 3, pp. 433-44, (2014) (PubMed).

Yao, Zhuang, Du, Cheng, Yang, Guan, Hu, Zhu, Christine, Shi, Hua: "Role of Fas-associated death domain-containing protein (FADD) phosphorylation in regulating glucose homeostasis: from proteomic discovery to physiological validation." in: Molecular & cellular proteomics : MCP, Vol. 12, Issue 10, pp. 2689-700, (2013) (PubMed).

Details zu PKM2

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Andere Bezeichnung: PKM2 (PKM2 Produkte)

Synonyme: CTHBP antikoerper, OIP3 antikoerper, PK3 antikoerper, PKM2 antikoerper, TCB antikoerper, THBP1 antikoerper, AA414905 antikoerper, AL024370 antikoerper, AL024424 antikoerper, Pk-2 antikoerper, Pk-3 antikoerper, Pk3 antikoerper, Pkm2 antikoerper, PKM12 antikoerper, PKM antikoerper, pyruvate kinase M1/2 antikoerper, pyruvate kinase, muscle antikoerper, PKM antikoerper, Pkm antikoerper

Hintergrund:

Synonyms: PK3, TCB, OIP3, PKM2, CTHBP, THBP1, HEL-S-3, Pyruvate kinase PKM, Cytosolic thyroid hormone-binding protein, Opa-interacting protein 3, OIP-3, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1, Tumor M2-PK, p58, PKM, PK2, Pyruvate kinase PKLR, Pyruvate kinase 1, R-type/L-type pyruvate kinase, Red cell/liver pyruvate kinase.

Background: Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

Gen-ID: 5315, 5313

UniProt: P14618, P30613

Pathways: Warburg Effekt