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GFP Antikörper (AA 25-120) (HRP)

GFP Reaktivität: Aequorea victoria, Aequorea coerulescens (Belt Jellyfish) WB, ELISA, IHC (fro), IHC (p) Wirt: Kaninchen Polyclonal HRP

Produktnummer ABIN729442

Datenblatt (PDF)

Target Alle GFP Antikörper anzeigen

GFP (Green Fluorescent Protein (GFP))
Bindungsspezifität
16

14

10

8

4

4

3

3

2

2

2

1

1

1

1

1

1

1
AA 25-120
Reaktivität
177

14

11

9
Aequorea victoria, Aequorea coerulescens (Belt Jellyfish)
Wirt
64

62

34

13

9

5

4

2
Kaninchen
Passende Sekundärantikörper
Klonalität
112

76
Polyklonal
Konjugat
83

12

11

9

5

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser GFP Antikörper ist konjugiert mit HRP
Applikation
147

79

48

34

28

27

23

21

13

13

13

12

11

5

4

4

4

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Kreuzreaktivität

Schwein, Tag

Kreuzreaktivität (Details)

Aequorea victoria, Aequorea coerulescens (belt jellyfish)

Aufreinigung

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from jellyfish Green Fluorecent protein

Isotyp

IgG

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Applikationshinweise

WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

1 μg/μL

Buffer

Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung

Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Lagerung

-20 °C

Informationen zur Lagerung

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit

12 months
Target

GFP (Green Fluorescent Protein (GFP))

Andere Bezeichnung

GFP (GFP Produkte)

Hintergrund

Synonyms: Green fluorescence protein, GRP, GFP TAG, GFP-TAG.
Background: Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.

UniProt

P42212