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PUMA Antikörper (AA 131-180)

Name: PUMA Antikörper (AA 131-180)
SKU: ABIN731528
Price: 384.62 EUR
Availability: InStock

BBC3 Reaktivität: Human, Ratte, Schwein WB, ELISA, IP, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Erianin induced mitochondrial apoptosis in liver cancer cells. (PUMA Antikörper (AA 131-180))

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti BBC3/PUMA Polyclonal Antibody, Unconjugated (ABIN731528) at 1:200 followed by conjugation to the secondary antibody and DAB staining (PUMA Antikörper (AA 131-180))

IHC

K562 lysates probed with PUMA Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C. (PUMA Antikörper (AA 131-180))

U87MG cells probed with Puma Polyclonal Antibody, Unconjugated at 1:50 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange). (PUMA Antikörper (AA 131-180))

FACS

4 Abbildungen

(3 Referenzen)

Produktnummer ABIN731528

Datenblatt (PDF)

Target Alle PUMA (BBC3) Antikörper anzeigen

PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
Bindungsspezifität
15

13

9

8

7

6

6

6

5

5

4

2

1

1

1

1

1

1

1

1
AA 131-180
Reaktivität
91

39

38

15
Human, Ratte, Schwein
Wirt
88

6

1
Kaninchen
Passende Sekundärantikörper
Klonalität
80

15
Polyklonal
Konjugat
47

6

5

5

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
Dieser PUMA Antikörper ist unkonjugiert
Applikation
76

33

29

20

15

13

12

12

8

7

5

2

2

2
Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Kreuzreaktivität

Human, Schwein, Ratte

Homologie

Mouse,Dog,Cow

Aufreinigung

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human PUMA

Isotyp

IgG

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BBC3 Reaktivität: Human WB, FACS, IHC (p), IF Wirt: Kaninchen Polyclonal RB01353 unconjugated

anti-BCL2 Binding Component 3 (BBC3) (AA 119-154) antibody
BBC3 Reaktivität: Human WB, ELISA, IHC, FACS, IF Wirt: Kaninchen Polyclonal unconjugated

anti-BCL2 Binding Component 3 (BBC3) (N-Term) antibody
BBC3 Reaktivität: Human WB, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-BCL2 Binding Component 3 (BBC3) (C-Term) antibody (PE)
BBC3 Reaktivität: Human WB, ICC, IF Wirt: Kaninchen Polyclonal PE

anti-BCL2 Binding Component 3 (BBC3) (C-Term) antibody (PerCP)
BBC3 Reaktivität: Human WB, ICC, IF Wirt: Kaninchen Polyclonal PerCP

anti-BCL2 Binding Component 3 (BBC3) (N-Term) antibody (Biotin)
BBC3 Reaktivität: Human WB, IHC Wirt: Kaninchen Polyclonal Biotin

anti-BCL2 Binding Component 3 (BBC3) (BH3 Domain), (C-Term) antibody
BBC3 Reaktivität: Human, Maus WB, FACS, IHC (p), IF, EIA Wirt: Kaninchen Polyclonal unconjugated

anti-BCL2 Binding Component 3 (BBC3) (AA 109-137) antibody
BBC3 Reaktivität: Human WB, IHC (p) Wirt: Kaninchen Polyclonal RB33714 unconjugated

anti-BCL2 Binding Component 3 (BBC3) (AA 98-152) antibody
BBC3 Reaktivität: Human WB, ELISA Wirt: Maus Monoclonal 5D10 unconjugated

Applikationshinweise

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
IP(1-2 μg)

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit

12 months
Su, Shi, Yan, Xi, Su: "Anticancer bioactive peptides suppress human colorectal tumor cell growth and induce apoptosis via modulating the PARP-p53-Mcl-1 signaling pathway." in: Acta pharmacologica Sinica, Vol. 36, Issue 12, pp. 1514-9, (2016) (PubMed).

Qiu, Cai, Gao, Gu, Liu: "A small peptide derived from p53 linker region can resume the apoptotic activity of p53 by sequestering iASPP with p53." in: Cancer letters, Vol. 356, Issue 2 Pt B, pp. 910-7, (2014) (PubMed).

Zhang, Liu, Xia, Zhang, Zhang, Zhao, Chao, Jiang, Jiang: "Epithelial-mesenchymal transition is necessary for acquired resistance to cisplatin and increases the metastatic potential of nasopharyngeal carcinoma cells." in: International journal of molecular medicine, Vol. 33, Issue 1, pp. 151-9, (2013) (PubMed).
Target

PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Andere Bezeichnung

PUMA (BBC3 Produkte)

Synonyme

BBC3 antikoerper, Puma antikoerper, JFY-1 antikoerper, JFY1 antikoerper, PUMA antikoerper, PUMA/JFY1 antikoerper, BCL2 binding component 3 antikoerper, Bcl-2 binding component 3 antikoerper, BBC3 antikoerper, Bbc3 antikoerper, bbc3 antikoerper

Hintergrund

Synonyms: JFY1, PUMA, JFY-1, Bcl-2-binding component 3, p53 up-regulated modulator of apoptosis, BBC3
Background: Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Isoform 3 fails to show any growth-inhibitory or apoptotic activity.

Gen-ID

27113

UniProt

Q96PG8

Pathways

p53 Signalweg, Positive Regulation of Endopeptidase Activity