LEF1 Antikörper (AA 331-399)

Produktnummer ABIN735248

Produktdetails anti-LEF1 Antikörper

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Bindungsspezifität: AA 331-399

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LEF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Dog,Cow,Pig,Chicken,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human LEF-1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-LEF1 Antikörper (ABIN735248)

Xu, Zhao, Zhang, Xu, Yang, Wang, Liu: "Resveratrol Attenuates Hyperoxia-induced Oxidative Stress, Inflammation and Fibrosis and Suppresses Wnt/?-catenin Signaling in Lungs of Neonatal Rats." in: Clinical and experimental pharmacology & physiology, (2015) (PubMed).

Wang, Xie, Xie, Wan, Ma, Qi, Yang: "MiR-27a regulates Wnt/beta-catenin signaling through targeting SFRP1 in glioma." in: Neuroreport, Vol. 26, Issue 12, pp. 695-702, (2015) (PubMed).

Song, Man, Wang, Bai, Wei, Liu, Liu, Gu, Wang: "The Regenerating Spinal Cord of Gecko Maintains Unaltered Expression of ?-Catenin Following Tail Amputation." in: Journal of molecular neuroscience : MN, (2014) (PubMed).

Details zu LEF1

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Andere Bezeichnung: LEF-1 (LEF1 Produkte)

Synonyme: LEF1 antikoerper, 3000002B05 antikoerper, AI451430 antikoerper, Lef-1 antikoerper, LEF-1 antikoerper, TCF10 antikoerper, TCF1ALPHA antikoerper, TCF7L3 antikoerper, lymphoid enhancer binding factor 1 antikoerper, lymphoid enhancer-binding factor 1 antikoerper, lymphoid enhancer factor XLEF-1B antikoerper, LEF1 antikoerper, lef1 antikoerper, Lef1 antikoerper

Hintergrund:

Synonyms: LEF-1, TCF1, TCF7L3, TCF1ALPHA, Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha, TCF1-alpha, LEF1

Background: Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP3. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.

Gen-ID: 51176

UniProt: Q9UJU2

Pathways: WNT Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding