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Neuroglobin Antikörper (AA 51-151)

Name: Neuroglobin Antikörper (AA 51-151)
SKU: ABIN735488
Price: 384.62 EUR
Availability: InStock

NGB Reaktivität: Ratte ELISA, IF (cc), IF (p), IHC (fro), IHC (p) Wirt: Kaninchen Polyclonal unconjugated

Formalin-fixed and paraffin embedded human brain tissue labeled with Anti-NGB/neuroglobin, Unconjugated (ABIN735488) followed by conjugation to the secondary antibody (Neuroglobin Antikörper (AA 51-151))

IHC

Paraformaldehyde-fixed, paraffin embedded rat brain, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with NGB/neuroglobin Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining (Neuroglobin Antikörper (AA 51-151))

IHC (p)

2 Abbildungen

Produktnummer ABIN735488

Datenblatt (PDF)

Target Alle Neuroglobin (NGB) Antikörper anzeigen

Neuroglobin (NGB)
Bindungsspezifität
14

9

7

6

1

1
AA 51-151
Reaktivität
34

18

3
Ratte
Wirt
45

5

1
Kaninchen
Passende Sekundärantikörper
Klonalität
47

4
Polyklonal
Konjugat
17

5

5

5

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser Neuroglobin Antikörper ist unkonjugiert
Applikation
25

24

14

12

12

4

3

3

2

2

2

1

1
ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Kreuzreaktivität

Ratte

Homologie

Human,Mouse,Cow,Pig,Rabbit

Aufreinigung

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from mouse NGB

Isotyp

IgG

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NGB Reaktivität: Human ELISA Wirt: Kaninchen Polyclonal Biotin

Applikationshinweise

ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit

12 months
Target

Neuroglobin (NGB)

Andere Bezeichnung

NGB/neuroglobin (NGB Produkte)

Synonyme

NGB antikoerper, ngb antikoerper, neuroglobin antikoerper, neuroglobin L homeolog antikoerper, NGB antikoerper, Ngb antikoerper, ngb antikoerper, ngb.L antikoerper

Hintergrund

Synonyms: Neuroglobin, Ngb
Background: Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes. The deoxygenated form exhibits nitrite reductase activity inhibiting cellular respiration via NO-binding to cytochrome c oxidase. Involved in neuroprotection during oxidative stress. May exert its anti-apoptotic activity by acting to reset the trigger level of mitochondrial cytochrome c release necessary to commit the cells to apoptosis.

Gen-ID

64242

UniProt

Q9ER97