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K-RAS Antikörper (AA 25-130)

Name: K-RAS Antikörper (AA 25-130)
SKU: ABIN736671
Price: 384.62 EUR
Availability: InStock

KRAS Reaktivität: Human, Ratte, Maus WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Formalin-fixed and paraffin embedded: human endometrium tissue labeled with Anti-KRAS Polyclonal Antibody (ABIN736671), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining (K-RAS Antikörper (AA 25-130))

IHC

Paraformaldehyde-fixed, paraffin embedded Mouse Kidney tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30min, Antibody incubation with KRAS Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining (K-RAS Antikörper (AA 25-130))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded mouse kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30min, Antibody incubation with KRAS Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37°C, followed by a conjugated streptavidin at 1:500 for 40 minutes and DAPI staining of the nuclei. (K-RAS Antikörper (AA 25-130))

IF (p)

3 Abbildungen

Produktnummer ABIN736671

Datenblatt (PDF)

Target Alle K-RAS (KRAS) Antikörper anzeigen

K-RAS (KRAS) (GTPase Kras (KRAS))
Bindungsspezifität
15

11

8

6

6

4

2

2

2

1

1

1

1

1

1

1
AA 25-130
Reaktivität
61

38

31

5

4

3

3

3

3

3

3

2

2

2

2

1

1

1
Human, Ratte, Maus
Wirt
63

11
Kaninchen
Passende Sekundärantikörper
Klonalität
62

12
Polyklonal
Konjugat
31

7

4

4

3

3

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser K-RAS Antikörper ist unkonjugiert
Applikation
62

30

22

13

12

12

10

5

5

4

2

1
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Kreuzreaktivität

Human, Maus, Ratte

Aufreinigung

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human K-ras

Isotyp

IgG

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KRAS Reaktivität: Human WB, ELISA, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

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KRAS Reaktivität: Human WB, IHC Wirt: Kaninchen Polyclonal unconjugated

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KRAS Reaktivität: Human, Ratte, Maus, Hund, Zebrafisch (Danio rerio), Kaninchen, Pferd, Meerschweinchen, Rind (Kuh), Saccharomyces cerevisiae WB Wirt: Kaninchen Polyclonal unconjugated

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KRAS Reaktivität: Human WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated

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KRAS Reaktivität: Human WB, ELISA Wirt: Maus Monoclonal 1E4-1B10 unconjugated

anti-GTPase Kras (KRAS) (C-Term) antibody
KRAS Reaktivität: Human, Ratte, Maus WB, ELISA, IHC Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit

12 months
Target

K-RAS (KRAS) (GTPase Kras (KRAS))

Andere Bezeichnung

KRAS (KRAS Produkte)

Substanzklasse

Viral Protein

Hintergrund

Synonyms: NS, NS3, CFC2, KRAS1, KRAS2, RASK2, KI-RAS, C-K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, GTPase KRas, K-Ras 2, c-Ki-ras, KRAS
Background: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation (PubMed:23698361, PubMed:22711838).

Gen-ID

3845

UniProt

P01116