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CDC25B Antikörper (pSer186)

Name: CDC25B Antikörper (pSer186)
SKU: ABIN756967
Price: 384.62 EUR
Availability: InStock

CDC25B Reaktivität: Maus WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti phospho-Cdc25B (Ser186) Polyclonal Antibody, Unconjugated (ABIN756967) at 1:200 followed by conjugation to the secondary antibody and DAB staining (CDC25B Antikörper (pSer186))

IHC

Paraformaldehyde-fixed, paraffin embedded mouse brain, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-Cdc25B (Ser186) Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary for 90 minutes and DAB staining. (CDC25B Antikörper (pSer186))

IHC (p)

Antigen: 0. (CDC25B Antikörper (pSer186))

ELISA

L1 rat brain lysates L2 mouse embryo lysates probed with Anti phospho-Cdc25B (Ser186) Polyclonal Antibody, Unconjugated (ABIN756967) at 1:200 overnight at 4 °C. (CDC25B Antikörper (pSer186))

SDS

4 Abbildungen

Produktnummer ABIN756967

Datenblatt (PDF)

Target Alle CDC25B Antikörper anzeigen

CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))
Bindungsspezifität
28

26

23

15

14

12

9

9

8

8

6

6

4

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer186
Reaktivität
145

116

55

7

7

4

4

3

3

2
Maus
Wirt
198

2
Kaninchen
Passende Sekundärantikörper
Klonalität
189

11
Polyklonal
Konjugat
73

15

12

11

5

5

5

5

5

5

5

5

5

5

5

5

5

4

4

4

4

4

4
Dieser CDC25B Antikörper ist unkonjugiert
Applikation
169

75

51

51

36

30

16

11

5

4

2

1

1
Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Kreuzreaktivität

Maus

Homologie

Human,Rat

Aufreinigung

Purified by Protein A.

Immunogen

KLH conjugated synthetic phosphopeptide derived from human Cdc25B around the phosphorylation site of Ser186

Isotyp

IgG
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer353) antibody
CDC25B Reaktivität: Human, Maus WB, ELISA, IHC (p) Wirt: Kaninchen Polyclonal unconjugated

anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (AA 331-360) antibody
CDC25B Reaktivität: Human WB, IHC (p) Wirt: Kaninchen Polyclonal RB07936 unconjugated

anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer323) antibody
CDC25B Reaktivität: Human, Maus, Ratte WB, ELISA, IHC, IF, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (AA 1-195) antibody
CDC25B Reaktivität: Human WB, ELISA, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer323) antibody
CDC25B Reaktivität: Maus WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer149) antibody
CDC25B Reaktivität: Human, Maus WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer353) antibody
CDC25B Reaktivität: Human, Maus WB, ELISA, IHC, IF, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer323) antibody
CDC25B Reaktivität: Human, Maus, Ratte WB, ELISA Wirt: Kaninchen Polyclonal unconjugated

anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer187) antibody
CDC25B Reaktivität: Human WB, IHC (p) Wirt: Kaninchen Polyclonal RB26741 unconjugated

anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer353) antibody
CDC25B Reaktivität: Human WB, IHC (p) Wirt: Kaninchen Polyclonal RB7935 unconjugated

Applikationshinweise

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit

12 months
Target

CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))

Andere Bezeichnung

Cdc25B (CDC25B Produkte)

Synonyme

CDC25B antikoerper, AI604853 antikoerper, cell division cycle 25B antikoerper, cdc25b antikoerper, CDC25B antikoerper, Cdc25b antikoerper

Hintergrund

Synonyms: M-phase inducer phosphatase 2, Dual specificity phosphatase Cdc25B, CDC25B, CDC25HU2
Background: Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.

Gen-ID

994

UniProt

P30305

Pathways

Zellzyklus, M Phase, Autophagie