MYCN Antikörper (AA 401-464)
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- Target Alle MYCN Antikörper anzeigen
- MYCN (N-Myc Proto-Oncogene Protein (MYCN))
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Bindungsspezifität
- AA 401-464
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Reaktivität
- Human, Maus
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser MYCN Antikörper ist unkonjugiert
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Applikation
- ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Homologie
- Rat,Cow,Pig
- Aufreinigung
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from human n-Myc
- Isotyp
- IgG
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- Applikationshinweise
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ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- by
- Alamo Laboratories Inc
- No.
- #029800
- Datum
- 21.08.2014
- Antigen
- Chargennummer
- 120917
- Validierte Anwendung
- Western Blotting
- Positivkontrolle
- MCF7 cells (variant MCF7/VP)00284-0/pdf)
- Negativkontrolle
- SK-N-SH cells - RNA
- Bewertung
- A strong band was observed in the positive control sample at the correct molecular weight. An additional band was also observed in the positive sample at a higher molecular weight which may represent non-specific binding. No bands were observed in the negative control sample.
- Primärantikörper
- Antigen: Mycn (MYCN)
- Catalog number: ABIN760676
- Lot number: 120917
- Antibody Dilution: 1:200
- Sekundärantikörper
- Antigen: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate
- Lot number: L170-6515
- Antibody Dilution: 1:10,000
- Full Protocol
- 1. The cell extracts were heated at 95°C for 5 minutes in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol.
- 2. 15 μl of heated culture-media were loaded and resolved on 8-16% SDS-polyacrylamide gel.
- 3. The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
- 4. Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
- 5. The PVDF membrane was incubated with 25 ml of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) BSA at room temperature for 1 hour.
- 6. The membrane was rinsed with TBST once.
- 7. The membrane was immersed with the protein side up in the primary antibody solution in TBST containing 5% (W/V) BSA and incubated for 13 hours at 4°C.
- 8. The membrane was rinsed in TBST thrice for 5 minutes each.
- 9. The membrane was incubated in the HRP-conjugated secondary antibody solution in TBST containing 5% (W/V) BSA and incubated for 1 hour at room temperature (~26°C) with gentle agitation.
- 10. The membrane was rinsed thrice TBST thrice for 5 minutes each.
- 11. The membrane was rinsed in TBS twice for 30 seconds each.
- 12. Signals were detected with ECL-2 Substrate. The blot was scanned for 45 minutes.
- 13. The membrane was rinsed three times TBST.
- 14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 minutes each.
- 15. The membrane was washed in TBST 2 times for 10 minutes each.
- 16. Repeated Steps 5-12 with the loading control antibody (for Anti-actin) and its matching secondary antibody.
- Anmerkungen
- - No experimental challenges noted.
Validierung #029800 (Western Blotting)ValidierungsbilderProtokoll -
- Format
- Liquid
- Konzentration
- 1 μg/μL
- Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
- Konservierungsmittel
- ProClin
- Vorsichtsmaßnahmen
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Haltbarkeit
- 12 months
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Serum-circulating miRNAs predict neuroblastoma progression in mouse model of high-risk metastatic disease." in: Oncotarget, Vol. 7, Issue 14, pp. 18605-19, (2016) (PubMed).
: "
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Serum-circulating miRNAs predict neuroblastoma progression in mouse model of high-risk metastatic disease." in: Oncotarget, Vol. 7, Issue 14, pp. 18605-19, (2016) (PubMed).
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- Target
- MYCN (N-Myc Proto-Oncogene Protein (MYCN))
- Andere Bezeichnung
- N-Myc (MYCN Produkte)
- Synonyme
- MODED antikoerper, MYCNOT antikoerper, N-myc antikoerper, NMYC antikoerper, ODED antikoerper, bHLHe37 antikoerper, Nmyc antikoerper, Nmyc-1 antikoerper, Nmyc1 antikoerper, c-nmyc antikoerper, Nmuc1 antikoerper, fb57a02 antikoerper, nmyc1 antikoerper, wu:fb57a02 antikoerper, zgc:85706 antikoerper, XN-myc antikoerper, XNmyc antikoerper, mycn antikoerper, mycn-a antikoerper, mycn-b antikoerper, mycna antikoerper, xN-myc1 antikoerper, MYCN proto-oncogene, bHLH transcription factor antikoerper, v-myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog antikoerper, v-myc avian myelocytomatosis viral related oncogene, neuroblastoma derived antikoerper, olfactory receptor 8S1 antikoerper, MYCN proto-oncogene, bHLH transcription factor S homeolog antikoerper, MYCN antikoerper, Mycn antikoerper, LOC515887 antikoerper, mycn antikoerper, mycn.S antikoerper
- Hintergrund
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This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. [provided by RefSeq, Jul 2008].
Subcellular location: Nucleus
Synonyms: NMYC, ODED, MODED, N-myc, bHLHe37, N-myc proto-oncogene protein, Class E basic helix-loop-helix protein 37, MYCN
- Gen-ID
- 4613
- UniProt
- P04198
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