POU2AF1 Antikörper (C-Term)

Produktnummer ABIN768553

Produktdetails anti-POU2AF1 Antikörper

Target: POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))

Bindungsspezifität: C-Term

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser POU2AF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Verwendungszweck: POU2AF1

Sequenz: DSDAYALNHT LSVE

Kreuzreaktivität: Rind (Kuh), Hund, Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Immunogen: Peptide with sequence C-DSDAYALNHTLSVE, from the C Terminus of the protein sequence according to NP_006226.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Spleen shows nuclear staining in the germinal center with stronger nucleoli staining in some of the B cells. Recommended concentration, 3-5 μg/mL.

Western Blot: In transfected HEK293 transiently expressing full-length Human POU2AF1 (myc and DYKDDDDK tagged), a band of approx. 38 kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag

Peptide ELISA: antibody detection limit dilution 1:8000.

Kommentare:

Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email sales@everestbiotech.com to request datasheet, pricing and delivery information.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu POU2AF1

Target: POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))

Andere Bezeichnung: POU2AF1 (POU2AF1 Produkte)

Synonyme: BOB1 antikoerper, OBF-1 antikoerper, OBF1 antikoerper, OCAB antikoerper, BOB-1 antikoerper, BOB.1 antikoerper, Bob-1 antikoerper, Bob1 antikoerper, OBF.1 antikoerper, OCA-B antikoerper, POU class 2 associating factor 1 antikoerper, POU domain, class 2, associating factor 1 antikoerper, POU2AF1 antikoerper, Pou2af1 antikoerper

Hintergrund: POU2AF1, POU class 2 associating factor 1, BOB1, OBF-1, OBF1, OCAB, POU domain, class 2, associating factor 1

Molekulargewicht: 38kDa

Gen-ID: 5450

NCBI Accession: NP_006226