Caspase 3 Antikörper (Internal Region)
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- Target Alle Caspase 3 (CASP3) Antikörper anzeigen
- Caspase 3 (CASP3)
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Bindungsspezifität
- Internal Region
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Reaktivität
- Human, Maus, Ratte
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Dieser Caspase 3 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
- Verwendungszweck
- Caspase 3
- Sequenz
- RDVSKEDHSK RS
- Spezifität
- This antibody is expected to recognise both reported isoforms (NP_004337.2 and NP_116786.1).
- Kreuzreaktivität
- Hund, Human, Maus, Ratte
- Aufreinigung
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Güteklasse
- Verified
- Immunogen
- Peptide with sequence C-RDVSKEDHSKRS, from the internal region of the protein sequence according to NP_004337.2, NP_116786.1.
- Isotyp
- IgG
- Top Product
- Discover our top product CASP3 Primärantikörper
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- Applikationshinweise
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Western Blot: Approx 32 kDa band observed in lysates of cell line MOLT4 and in Mouse Liver lysates (calculated MW of 31.6 kDa according to Human NP_004337.2 and 31.4 kDa according to Mouse NP_033940.1, NP_001271338.1). An additional band of 65 kDa was also
Peptide ELISA: antibody detection limit dilution 1:32000.
- Kommentare
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 cells. Recommended concentration: 10μg/ml. This antibody has been successfully used in ICC on Rat: Hóngyi Zhào et al. (2018) PMID: 29853981.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Minimize freezing and thawing.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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Troxerutin and Cerebroprotein Hydrolysate Injection Protects Neurovascular Units from Oxygen-Glucose Deprivation and Reoxygenation-Induced Injury In Vitro." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2018, pp. 9859672, (2018) (PubMed).
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Troxerutin and Cerebroprotein Hydrolysate Injection Protects Neurovascular Units from Oxygen-Glucose Deprivation and Reoxygenation-Induced Injury In Vitro." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2018, pp. 9859672, (2018) (PubMed).
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- Target
- Caspase 3 (CASP3)
- Andere Bezeichnung
- CASP3 (CASP3 Produkte)
- Synonyme
- CPP32 antikoerper, CPP32B antikoerper, SCA-1 antikoerper, A830040C14Rik antikoerper, AC-3 antikoerper, Apopain antikoerper, CC3 antikoerper, Caspase-3 antikoerper, Lice antikoerper, Yama antikoerper, mldy antikoerper, xcpp32 antikoerper, casp3 antikoerper, zgc:100890 antikoerper, CASP-3 antikoerper, caspase-3 antikoerper, caspase 3 antikoerper, caspase 3 S homeolog antikoerper, caspase 3, apoptosis-related cysteine peptidase a antikoerper, caspase 3, apoptosis-related cysteine peptidase antikoerper, CASP3 antikoerper, Casp3 antikoerper, casp3.S antikoerper, casp3a antikoerper
- Hintergrund
- CASP3, caspase 3, caspase 3, apoptosis-related cysteine protease, HGNC:1504, caspase 3, apoptosis-related cysteine peptidase, CPP32, CPP32B, SCA-1, PARP cleavage protease, SREBP cleavage activity 1, Yama, apopain, cysteine protease CPP32, procaspase3
- Molekulargewicht
- 31.6kDa according to NP_004337.2
- Gen-ID
- 836
- NCBI Accession
- NP_004337, NP_116786
- Pathways
- Apoptose, Caspase Kaskade in der Apoptose, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation
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