Albumin Antikörper (HRP)

Produktnummer ABIN784423

Produktdetails anti-Albumin Antikörper (HRP)

Target: Albumin (ALB)

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Albumin Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Radioimmunoassay (RIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: Tested in Immunoelectrophoresis and Double Radial Immunodiffusion against pooled normal Mouse serum and purified Mouse Albumin. One characteristic precipitin line is obtained against pooled normal Mouse serum using different antigen/antibody concentration ratio's. Precipitin lines against normal Mouse serum and purified Mouse Albumin give a reaction of full identity. Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum has been tested for cross-reactivity in Double Radial Immunodiffusion with the following results: Bovine (-), Chicken (-), Canine (-), Goat (-), Guinea Pig (++), Hamster (+), Horse (+), Human (+), Monkey (+), Rat (++), Sheep (-), Swine (+). A negative cross-reaction in Double Radial Immunodiffusion does not exclude some reaction in more sensitive techniques.

Produktmerkmale: Molar Ratio: Enzyme/IgG ~1.7

Aufreinigung: Sequential Precipitation, Ion Exchange Chromatography and Affinity Chromatography

Immunogen: Albumin is isolated from Mouse serum by sequential precipitation and purified by ion exchange chromatography and affinity chromatography. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore by adding 1 mL of sterile distilled water

Konzentration: 10 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Details zu Albumin

Target: Albumin (ALB)

Andere Bezeichnung: Albumin (ALB Produkte)

Hintergrund: Albumin is a soluble, monomeric protein which comprises about one half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.Synonyms: ALB, BSA, HSA, Serum Albumin

Gen-ID: 11657

NCBI Accession: NP_033784

Pathways: Lipid Metabolism