CD4 Antikörper (Biotin)

Produktnummer ABIN793379

Produktdetails anti-CD4 Antikörper (Biotin)

Target: CD4

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD4 Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS)

Spezifität: Human CD4

Aufreinigung: Protein A/G purified

Sterilität: Sterile filtered

Immunogen: Human peripheral blood T lymphocytes.

Klon: 7E14

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: Flo

Usage: PBMC: Add 10 μL of antibody/10^6 PBMC in 100 μL PBS. Mix gently and incubate for 15 minutes at 2° to 8 °C. Wash twice with PBS and analyze or fix with 0.5 % v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 μL of antibody/100 μL of whole blood. Mix gently and incubate for 15 minutes at room temperature 20 °C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5 % v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5 +: Use 5 μL of solution, rather than 10 μL as above. Excites at 488nm and emits at 670nm. Store protected from light. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.08 % sodium azide, 0.2 % carrier protein, sterile-filtered

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4°C. Do not freeze.

Details zu CD4

Target: CD4

Andere Bezeichnung: CD4 (CD4 Produkte)

Synonyme: cd4 antikoerper, L3T4 antikoerper, Ly-4 antikoerper, CD4mut antikoerper, W3/25 antikoerper, p55 antikoerper, CD4 molecule antikoerper, carcinoembryonic antigen-related cell adhesion molecule 5 antikoerper, T-cell surface glycoprotein CD4 antikoerper, CD4 antigen antikoerper, Cd4 molecule antikoerper, CD4 antikoerper, LOC100125525 antikoerper, cd4 antikoerper, Cd4 antikoerper

Hintergrund: Name/Gene ID: CD4

Synonyms: CD4, CD4 antigen, CD4 receptor, CD4mut, CD4 antigen (p55), CD4 Molecule

Gen-ID: 920

UniProt: P01730

Pathways: T-Zell Rezeptor Signalweg, Maintenance of Protein Location, CXCR4-mediated Signaling Events