HSPA4 Antikörper

Produktnummer ABIN863194

Produktdetails anti-HSPA4 Antikörper

Target: HSPA4 (Heat Shock 70kDa Protein 4 (HSPA4))

Reaktivität: Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HSPA4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität: Detects ~110 kDa.

Kreuzreaktivität: Rind (Kuh), Hamster, Human, Affe, Maus, Ratte, Saccharomyces cerevisiae, Hai, Schaf

Aufreinigung: Protein A Purified

Immunogen: Synthetic peptide derived from the sequence of hamster HSP110, sequence identical to human and mouse

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• IHC (1:100)
• optimal dilutions for assays should be determined by the user.

Kommentare:

1 μg/ml of ABIN863193 was sufficient for detection of HSP110 in 10 μg of human cell line mixed lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Referenzen für anti-HSPA4 Antikörper (ABIN863194)

Eng, Reed, Kokolus, Repasky: "Housing temperature influences the pattern of heat shock protein induction in mice following mild whole body hyperthermia." in: International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group, Vol. 30, Issue 8, pp. 540-6, (2014) (PubMed).

Details zu HSPA4

Target: HSPA4 (Heat Shock 70kDa Protein 4 (HSPA4))

Andere Bezeichnung: HSP110 (HSPA4 Produkte)

Hintergrund: HSP110 belongs to a family of large stress proteins known as the HSP110/SSE Family. The proteins in this family are the most distantly known relatives of the well studied HSP70 family. They share 30-33 % amino acid identity, mostly in the conserved ATP-binding domain (1). HSP110 cooperates with HSP70 in protein folding in the eukaryotic cytosol (2). In mammals, HSP110 is constitutively expressed, but exhibits particularly high levels in the brain. Both HSP70 and HSP110 are elevated after cerebral ischemia. Recent studies demonstrate that the protective effects of HSP110 deficiency in cerebral ischemia may partly be mediated by an increase in the chaperone activity of HSP70 (3). Studies also suggest that HSP110 can be used in heat shock protein-based cancer immunotherapy (4).

Gen-ID: 10808

NCBI Accession: NP_006635

UniProt: Q92599