GFP Tag Antikörper

Produktnummer ABIN871608

Produktdetails anti-GFP Tag Antikörper

Target: GFP Tag

Reaktivität: Aequorea victoria

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GFP Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Spezifität: GFP Antibody detects GFP and GFP fusion proteins.

Anwendungsinformationen

Applikationshinweise: Western blotting 1:2000. Immunoprecipitation 1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Lagerung: -20 °C

Details zu GFP Tag

Target: GFP Tag

Andere Bezeichnung: GFP-Tag (GFP Tag Produkte)

Substanzklasse: Tag

Hintergrund: This polyclonal antibody is produced by immunizing animals with a synthetic peptide (KLH-coup s ide pled) corresponding to the carboxy terminus EGFP. The green fluorescent prote (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequore victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.