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SIRT4 Antikörper (AA 51-150)

Name: SIRT4 Antikörper (AA 51-150)
SKU: ABIN872709
Price: 357.7 EUR
Availability: InStock

Dieses Anti-SIRT4-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von SIRT4 in WB, ELISA und IHC (p). Geeignet für Maus und Ratte. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.

Mouse cerebrum lysates probed with SIRT4 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C. (SIRT4 Antikörper (AA 51-150))

Paraformaldehyde-fixed, paraffin embedded rat stomach tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-SIRT4 Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining (SIRT4 Antikörper (AA 51-150))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded rat kidney tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-SIRT4 Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining (SIRT4 Antikörper (AA 51-150))

IHC (p)

Mouse liver lysates probed with SIRT4 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C. (SIRT4 Antikörper (AA 51-150))

4 Abbildungen

(2 Referenzen)

Produktnummer ABIN872709

Kurzübersicht für SIRT4 Antikörper (AA 51-150) (ABIN872709)

Target

Alle SIRT4 Antikörper anzeigen

SIRT4 (Sirtuin 4 (SIRT4))

Reaktivität

Maus, Ratte

Wirt

Kaninchen

Klonalität

Polyklonal

Konjugat

Dieser SIRT4 Antikörper ist unkonjugiert

Applikation

Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

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Bindungsspezifität
12

8

7

3

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1
AA 51-150

Kreuzreaktivität

Maus, Ratte

Homologie

Human,Cow,Sheep,Pig,Horse,Rabbit,Zebrafish,Drosophila

Aufreinigung

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human SIRT4/SIR 2 like protein 4

Isotyp

IgG
anti-Sirtuin 4 (SIRT4) (AA 28-314) antibody
SIRT4 Reaktivität: Human WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Sirtuin 4 (SIRT4) (AA 60-255) antibody
SIRT4 Reaktivität: Ratte WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Sirtuin 4 (SIRT4) (C-Term) antibody
Verified SIRT4 Reaktivität: Human WB, IHC, ELISA Wirt: Ziege Polyclonal unconjugated

anti-Sirtuin 4 (SIRT4) (AA 215-314) antibody
SIRT4 Reaktivität: Human ELISA, ICC, FACS Wirt: Maus Monoclonal 6G6D2 unconjugated

anti-Sirtuin 4 (SIRT4) (Middle Region) antibody
SIRT4 Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Schwein, Pferd, Kaninchen, Schaf, Meerschweinchen WB, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-Sirtuin 4 (SIRT4) (AA 29-260) antibody
SIRT4 Reaktivität: Human WB, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-Sirtuin 4 (SIRT4) (AA 26-333) antibody
SIRT4 Reaktivität: Maus WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Sirtuin 4 (SIRT4) (AA 215-314) antibody
SIRT4 Reaktivität: Human WB, ELISA Wirt: Maus Monoclonal 1C8 unconjugated

anti-Sirtuin 4 (SIRT4) (AA 216-265) antibody
SIRT4 Reaktivität: Human, Hund, Schwein, Affe WB, IHC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated

anti-Sirtuin 4 (SIRT4) (N-Term) antibody
SIRT4 Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Schwein, Pferd, Kaninchen, Schaf, Meerschweinchen, Ziege, Zebrafisch (Danio rerio) WB Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit

12 months
Takumida, Takumida, Katagiri, Anniko: "Localization of sirtuins (SIRT1-7) in the aged mouse inner ear." in: Acta oto-laryngologica, pp. 1-12, (2015) (PubMed).

Takumida, Takumida, Anniko: "Localization of sirtuins in the mouse inner ear." in: Acta oto-laryngologica, Vol. 134, Issue 4, pp. 331-8, (2014) (PubMed).
Target

SIRT4 (Sirtuin 4 (SIRT4))

Andere Bezeichnung

SIRT4

Hintergrund

Synonyms: SIR2L4, NAD-dependent protein deacetylase sirtuin-4, NAD-dependent ADP-ribosyltransferase sirtuin-4, Regulatory protein SIR2 homolog 4, SIR2-like protein 4, SIRT4
Background: Acts both as NAD-dependent protein ADP-ribosyl transferase and NAD-dependent protein deacetylase. Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity. Acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1: expressed in response to DNA damage and negatively regulates anaplerosis by inhibiting GLUD1, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest. In response to mTORC1 signal, SIRT4 expression is repressed, promoting anaplerosis and cell proliferation. Acts as a tumor suppressor. Also acts as a NAD-dependent protein deacetylase: mediates deacetylation of 'Lys-471' of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis. Down-regulates insulin secretion.

Gen-ID

23409

UniProt

Q9Y6E7

Pathways

Negative Regulation of Hormone Secretion