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ALOX15B Antikörper (AA 13-110)

Name: ALOX15B Antikörper (AA 13-110)
SKU: ABIN873138
Price: 384.62 EUR
Availability: InStock

ALOX15B Reaktivität: Ratte ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Paraformaldehyde-fixed, paraffin embedded human prostate, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with ALOX15B Polyclonal Antibody, Unconjugated ) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining. (ALOX15B Antikörper (AA 13-110))

IHC (p)

Lane 1: mouse brain lysates Lane 2: mouse heart lysates probed with Anti ALOX15B/15 Lipoxygenase 2 Polyclonal Antibody, Unconjugated (ABIN873138) at 1:200 in 4 °C. (ALOX15B Antikörper (AA 13-110))

SDS

2 Abbildungen

Produktnummer ABIN873138

Datenblatt (PDF)

Target Alle ALOX15B Antikörper anzeigen

ALOX15B (Arachidonate 15-Lipoxygenase B (ALOX15B))
Bindungsspezifität
14

6

4

3

3

2

2

1

1

1

1

1

1

1

1
AA 13-110
Reaktivität
33

20

6

5

4

4

4

2

2

1
Ratte
Wirt
46

2
Kaninchen
Passende Sekundärantikörper
Klonalität
46

2
Polyklonal
Konjugat
24

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser ALOX15B Antikörper ist unkonjugiert
Applikation
22

13

12

12

9

5

5

3

2

2

1

1

1
ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Kreuzreaktivität

Ratte

Homologie

Human,Mouse,Cow,Pig,Horse,Rabbit

Aufreinigung

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human ALOX15B/15 Lipoxygenase 2

Isotyp

IgG

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anti-Arachidonate 15-Lipoxygenase B (ALOX15B) (AA 1-200) antibody
ALOX15B Reaktivität: Human WB, IF Wirt: Kaninchen Polyclonal unconjugated

anti-Arachidonate 15-Lipoxygenase B (ALOX15B) (AA 181-280) antibody
ALOX15B Reaktivität: Human WB, ELISA, IF Wirt: Maus Monoclonal 4A7 unconjugated

anti-Arachidonate 15-Lipoxygenase B (ALOX15B) (AA 1-200) antibody
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ALOX15B Reaktivität: Human WB, IHC (p) Wirt: Kaninchen Polyclonal RB17162 unconjugated

anti-Arachidonate 15-Lipoxygenase B (ALOX15B) antibody
ALOX15B Reaktivität: Human, Maus WB, IF Wirt: Kaninchen Polyclonal unconjugated

anti-Arachidonate 15-Lipoxygenase B (ALOX15B) (AA 1-676) antibody
ALOX15B Reaktivität: Human ELISA, IF Wirt: Kaninchen Polyclonal unconjugated

anti-Arachidonate 15-Lipoxygenase B (ALOX15B) (AA 1-200) antibody
ALOX15B Reaktivität: Human ELISA, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-Arachidonate 15-Lipoxygenase B (ALOX15B) (AA 401-450) antibody
ALOX15B Reaktivität: Human, Ratte, Maus, Rind (Kuh), Pferd, Kaninchen, Schwein, Hund, Meerschweinchen WB Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit

12 months
Target

ALOX15B (Arachidonate 15-Lipoxygenase B (ALOX15B))

Andere Bezeichnung

ALOX15B (ALOX15B Produkte)

Synonyme

15-LOX-2 antikoerper, ALOX15B antikoerper, arachidonate 15-lipoxygenase, type B antikoerper, arachidonate 15-lipoxygenase B antikoerper, ALOX15B antikoerper, Alox15b antikoerper, LOC100525835 antikoerper

Hintergrund

Synonyms: 15-LOX-2, Arachidonate 15-lipoxygenase B, 15-LOX-B, 15-lipoxygenase 2, Arachidonate 15-lipoxygenase type II, Linoleate 13-lipoxygenase 15-LOb, ALOX15B
Background: Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid/(15S)-HPETE. Also acts on linoleic acid to produce 13-hydroxyoctadecadienoic acid/13-HPODE. Has no detectable 8S-lipoxygenase activity but reacts with (8S)-HPETE to produce (8S,15S)-diHPETE. May regulate progression through the cell cycle and cell proliferation. May also regulate cytokine secretion by macrophages and therefore play a role in the immune response. May also regulate macrophage differentiation into proatherogenic foam cells.

Gen-ID

247

UniProt

O15296