RAPTOR Antikörper (pSer792) (AbBy Fluor® 350)

Produktnummer ABIN877075

Produktdetails anti-RAPTOR Antikörper (AbBy Fluor® 350)

Target: RAPTOR

Bindungsspezifität: pSer792

Reaktivität: Human, Maus, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAPTOR Antikörper ist konjugiert mit AbBy Fluor® 350

Applikation: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Kreuzreaktivität: Human, Maus, Zebrafisch (Danio rerio)

Homologie: Rat,Dog,Cow,Sheep,Pig,Horse,Chicken,Guinea Pig

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from mouse Raptor around the phosphorylation site of Ser792

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu RAPTOR

Target: RAPTOR

Andere Bezeichnung: Raptor (RAPTOR Produkte)

Synonyme: KOG1 antikoerper, Mip1 antikoerper, 4932417H02Rik antikoerper, Rap antikoerper, Raptor antikoerper, mKIAA1303 antikoerper, RGD1311784 antikoerper, regulatory associated protein of MTOR complex 1 antikoerper, regulatory associated protein of MTOR, complex 1 antikoerper, RPTOR antikoerper, Rptor antikoerper

Hintergrund:

Synonyms: ARaptor phospho S792, Raptor phospho Ser792, p-Raptor Ser792, p150 target of rapamycin TOR scaffold protein containing WD repeats, P150 target of rapamycin TOR-scaffold protein, Regulatory Associated Protein of mTOR, KIAA1303, RPTOR_HUMAN, Regulatory-associated protein of mTOR, p150 target of rapamycin TOR-scaffold protein.

Background: mTOR controls cell growth, in part by regulating p70 S6 kinase alpha (p70alpha) and eukaryotic initiation factor 4E binding protein 1 (4EBP1). Raptor is a 150 kDa mTOR binding protein that also binds 4EBP1 and p70alpha. The binding of Raptor to mTOR is necessary for the mTOR-catalyzed phosphorylation of 4EBP1 in vitro, and it strongly enhances the mTOR kinase activity toward p70alpha. Rapamycin or amino acid withdrawal increases, whereas insulin strongly inhibits, the recovery of 4EBP1 and raptor on 7-methyl-GTP Sepharose. Partial inhibition of raptor expression by RNA interference (RNAi) reduces mTOR-catalyzed 4EBP1 phosphorylation in vitro. RNAi of C. elegans raptor yields an array of phenotypes that closely resemble those produced by inactivation of Ce-TOR. Thus, raptor is an essential scaffold for the mTOR-catalyzed phosphorylation of 4EBP1 and mediates TOR action in vivo.

Pathways: Warburg Effekt