Girdin Antikörper (AA 1401-1500) (AbBy Fluor® 647)

Produktnummer ABIN882970

Produktdetails anti-Girdin Antikörper (AbBy Fluor® 647)

Target: Girdin (CCDC88A) (Coiled-Coil Domain Containing 88A (CCDC88A))

Bindungsspezifität: AA 1401-1500

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Girdin Antikörper ist konjugiert mit AbBy Fluor® 647

Applikation: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Kreuzreaktivität: Human

Homologie: Mouse,Rat,Dog,Horse,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human APE/Girdin

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu Girdin

Target: Girdin (CCDC88A) (Coiled-Coil Domain Containing 88A (CCDC88A))

Andere Bezeichnung: Girdin (CCDC88A Produkte)

Synonyme: APE antikoerper, GIRDIN antikoerper, GIV antikoerper, GRDN antikoerper, HkRP1 antikoerper, KIAA1212 antikoerper, A430106J12Rik antikoerper, AI848406 antikoerper, Ape antikoerper, C130096N06Rik antikoerper, C330012F17Rik antikoerper, D130005J21Rik antikoerper, Girdin antikoerper, Giv antikoerper, Grdn antikoerper, Hkrp1 antikoerper, DKFZp459N1815 antikoerper, RGD1306694 antikoerper, coiled-coil domain containing 88A antikoerper, coiled coil domain containing 88A antikoerper, CCDC88A antikoerper, Ccdc88a antikoerper

Hintergrund:

Synonyms: APE, APX, APE1, APEN, APEX, HAP1, REF1, DNA-(apurinic or apyrimidinic site) lyase, APEX nuclease, Apurinic-apyrimidinic endonuclease 1, AP endonuclease 1, APE-1, REF-1, Redox factor-1, APEX1

Background: Multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 in DNA repair and redox regulation of transcriptional factors. Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Does also incise at AP sites in the DNA strand of DNA/RNA hybrids, single-stranded DNA regions of R-loop structures, and single-stranded RNA molecules. Has a 3'-5' exoribonuclease activity on mismatched deoxyribonucleotides at the 3' termini of nicked or gapped DNA molecules during short-patch BER. Possesses a DNA 3' phosphodiesterase activity capable of removing lesions (such as phosphoglycolate) blocking the 3' side of DNA strand breaks. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Acts as a loading factor for POLB onto non-incised AP sites in DNA and stimulates the 5'-terminal deoxyribose 5'-phosphate (dRp) excision activity of POLB. Plays a role in the protection from granzymes-mediated cellular repair leading to cell death. Also involved in the DNA cleavage step of class switch recombination (CSR). On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. Involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs).

Gen-ID: 328

UniProt: P27695