Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)
Spezifität
JSB-1 reacts with a conserved cytoplasmic epitope of the human plasma membrane- associated 170-180 kDa glycoprotein, the expression of which is strongly correlated with the degree of multidrug resistance (MDR) derived MDR cell lines and human MDR cell lines, including cell lines derived from lung, ovaries and B cell lymphomas.
Reaktivität: Human
WB
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Can be used for the detection of MDR cells in human tumor samples. Flow Cytometry: Use at least 1:10. Fix cells in 10% (v/v) Lysing Solution, followed by primary antibody and anti-mouse-FITC. Immunocytochemistry: Use at least 1:20 (acetone fixed cell preparation) Immunohistochemistry: acetone-fixed frozen sections or formalin-fixed paraffin embedded tissues: Use at 1:20 dilution. Optimal staining results are obtained with routine 2-step ABC or APAAP methods using acetone-fixed cytocentrifuge preparations or cryostat sections. In case the B-5 fixative is used, paraffin-embedded tissue can also be stained with the antibody. Western blotting: Start optimizing working dilution at 1:10, followed by incubation with anti-mouse HRP.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
1 ml concentrated tissue culture supernatant (200 tests) at ~250 µg IgG/ml, with 1% BSA and 0.1% NaN3.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.