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P-Glycoprotein Antikörper

Reaktivität: Human, Hamster WB, IHC, FACS Wirt: Maus Monoclonal JSB-1 unconjugated
Produktnummer ABIN920710
  • Target Alle P-Glycoprotein Produkte
    P-Glycoprotein
    Reaktivität
    • 21
    • 10
    • 9
    • 1
    Human, Hamster
    Wirt
    • 16
    • 6
    Maus
    Klonalität
    • 14
    • 7
    • 1
    Monoklonal
    Konjugat
    • 15
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser P-Glycoprotein Antikörper ist unkonjugiert
    Applikation
    • 11
    • 6
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
    Spezifität
    JSB-1 reacts with a conserved cytoplasmic epitope of the human plasma membrane- associated 170-180 kDa glycoprotein, the expression of which is strongly correlated with the degree of multidrug resistance (MDR) derived MDR cell lines and human MDR cell lines, including cell lines derived from lung, ovaries and B cell lymphomas.
    Produktmerkmale
    Mouse myeloma (SP2/0) cells fused with immunized mouse (Balb/C) lymph node cells.
    Sterilität
    0.22 μm filtered
    Klon
    JSB-1
    Isotyp
    IgG1
  • Applikationshinweise
    Flow cytometry: Use at least a 1:10 dilution. Fix cells in 10% (v/v) Lysing Solution, followed by primary antibody and anti-mouse- FITC. Immunocytochemistry: Use at least a 1:20 dilution. (acetone-fixed cell preparation) Immunohistochemistry: acetone-fixed frozen sections or formalin-fixed, paraffin-embedded tissues: Use at a 1:20 dilution. Optimal staining results are obtained with routine 2-step ABC or APAAP methods using acetone-fixed cytocentrifuge preparations or cryostat sections. In case the B-5 fixative is used (see below), paraffin-embedded tissue can also be stained with the antibody. Western blot: Start optimizing working dilution at 1:10, followed by incubation with anti- mouse HRP. B-5 Fixative Procedure: Fix tissue in freshly prepared B-5 fixative: add 2 mL of 40% formalin to 20 mL of B-5 Stock Solution. Sections must be dezenkerized to remove mercuric pigment before immunostaining. Dezenkerize by placing slides with sections in Gram's Iodine solution for 5 min. at room temperature. Wash well in distilled water. Place slides in Sodium Thiosulphate solution for 5 min. at room temperature. Wash in running tap water for 3 min. Place in PBS for further immunochemical procedures. B-5 Stock Solution: 12 g mercuric chloride 2.5 g sodium acetate Dissolve in 200 mL distilled water. Gram's Iodine: 1 g I2 2 g KI Dissolve in 300 mL distilled water. KAMIYA BIOMEDICAL COMPANY Rev. 021507 PRODUCT DATA SHEET KAMIYA BIOMEDICAL COMPANY, Seattle, WA USA Tel: (206) 575-8068
    Fax: (206) 575-8094 www.k-assay.com LifeScience@k-assay.com
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    1 mL (200 tests) or 0.5 mL (100 tests) monoclonal antibody at ~250 µg IgG/mL in tissue culture supernatant with protein stabilizer and 0.1% sodium azide. Filtered through a 0.22 µm filter. Culture medium: RPMI-1640, supplemented with Nutridoma-SR. The medium does not contain serum or added enzymes.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
  • Target
    P-Glycoprotein
    Andere Bezeichnung
    MDR1 / P-Glycoprotein (P-Glycoprotein Produkte)
    Synonyme
    ABC20 antikoerper, CD243 antikoerper, CLCS antikoerper, GP170 antikoerper, MDR1 antikoerper, P-GP antikoerper, PGY1 antikoerper, Abcb1 antikoerper, Mdr1a antikoerper, ABCB1 antikoerper, ABCB4 antikoerper, CMDR1 antikoerper, xemdr antikoerper, ATP binding cassette subfamily B member 1 antikoerper, ATP binding cassette subfamily B member 1A antikoerper, ATP binding cassette subfamily B member 1 L homeolog antikoerper, ABCB1 antikoerper, Abcb1a antikoerper, abcb1.L antikoerper
    Hintergrund
    Synonyms: P-glycoprotein, MDR1, p170, ABCB1
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