Immunoprecipitation (IP), Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Dot Blot (DB)
Kreuzreaktivität
Human, Maus, Ratte (Rattus)
Kreuzreaktivität (Details)
Other species not studied.
Aufreinigung
Protein G affinity chromatography
Immunogen
BTAF1 antibody was raised in mouse using recombinant Btaf1 Rna Polymerase Ii, B-Tfiid Transcription Factor-Associated, 170 Kda (Mot1 Homolog, S. Cerevisiae) (Btaf1)
BTAF1
Reaktivität: Human
IP, WB
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
WB: 0.2-2 µg/mL, IP: 100-500 µg/sample, FC: 0.5-2 µg/sample, ICC: 2-100 µg/mL Optimal conditions should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
Lot specific
Buffer
BTAF1 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1 % bovine serum albumin (BSA) and 0.05 % sodium azide (NaN3).
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handhabung
Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store at 2-8 °C for up to one year. We recommend long term storage at -20 °C.
Initiation of transcription by RNA polymerase II requires the assistance of TATA box-binding protein (TBP, MIM 600075) and TBP-associated factors, or TAFs (e.g., TAF2B, MIM 604912), in 2 distinct complexes, TFIID and B-TFIID. The TFIID complex is composed of TBP and more than 8 TAFs. However, the majority of TBP is present in the B-TFIID complex, which is composed of TBP and TAFII170, also called TAF172, and has DNA-dependent ATPase activity. Synonyms: Monoclonal BTAF1 antibody, Anti-BTAF1 antibody, B-TFIID transcription factor-associated, MOT1 antibody, TAF172 antibody, KIAA0940 antibody, TAFII170 antibody, TAF(II)170 antibody.