Expected / apparent Molecular Weight of the Antigene: 25 / 25 kDa for Arabidopsis thaliana
Aufreinigung
serum
Immunogen
BSA-conjugated synthetic peptide derived from a highly conserved sequence of Lhb1 proteins from angiosperms (monocots and dicots) and gymnosperms, includingArabidopsis thaliana At1g29910 (Lhcb1.1), At1g29920 (Lhcb1.2), At1g29930 (Lhcb1.3, most expressed), At1g34430 (Lhcb1.4), and At1g34420 (Lhcb1.5)
Recommended Dilution: 1 : 2000, detected with standard ECL (WB), 1 : 100 - 1 : 500 (IG).
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
For reconstitution add 10 µL of sterile water
Handhabung
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Lagerung
-20 °C
Mazur, Sadowska, Kowalewska, Abratowska, Kalaji, Mostowska, Garstka, Krasnodębska-Ostręga: "Overlapping toxic effect of long term thallium exposure on white mustard (Sinapis alba L.) photosynthetic activity." in: BMC plant biology, Vol. 16, Issue 1, pp. 191, (2016) (PubMed).
Charuvi, Nevo, Shimoni, Naveh, Zia, Adam, Farrant, Kirchhoff, Reich: "Photoprotection conferred by changes in photosynthetic protein levels and organization during dehydration of a homoiochlorophyllous resurrection plant." in: Plant physiology, Vol. 167, Issue 4, pp. 1554-65, (2015) (PubMed).
Sobrino-Plata, Carrasco-Gil, Abadía, Escobar, Álvarez-Fernández, Hernández: "The role of glutathione in mercury tolerance resembles its function under cadmium stress in Arabidopsis." in: Metallomics : integrated biometal science, Vol. 6, Issue 2, pp. 356-66, (2014) (PubMed).
Schmied, Hedtke, Grimm: "Overexpression of HEMA1 encoding glutamyl-tRNA reductase." in: Journal of plant physiology, (2011) (PubMed).
Yin, Lundin, Bertrand, Nurmi, Solymosi, Kangasjarvi, Aro, Schoefs, Spetea: "Role of Thylakoid ATP/ADP Carrier in Photoinhibition and Photoprotection of Photosystem II in Arabidopsis." in: Plant physiology, (2010) (PubMed).
Yuan, Xu, Yuan, Chen, Du, Xu, Zhang, Guo, Zhao, Lin: "Light regulation to chlorophyll synthesis and plastid development of the chlorophyll-less golden-leaf privet." in: Journal of integrative plant biology, Vol. 52, Issue 9, pp. 809-16, (2010) (PubMed).
Munekage, Eymery, Rumeau, Cuina: "Elevated expression of PGR5 and NDH-H in bundle sheath chloroplasts in C4 flaveria species." in: Plant & cell physiology, Vol. 51, Issue 4, pp. 664-8, (2010) (PubMed).
Saito, Iino, Sonoike, Miwa, Higuchi: "Remodeling of the major light-harvesting antenna protein of PSII protects the young leaves of barley (Hordeum vulgare L.) from photoinhibition under prolonged iron deficiency." in: Plant & cell physiology, Vol. 51, Issue 12, pp. 2013-30, (2010) (PubMed).
Tikkanen, Nurmi, Suorsa, Danielsson, Mamedov, Styring, Aro: "Phosphorylation-dependent regulation of excitation energy distribution between the two photosystems in higher plants." in: Biochimica et biophysica acta, Vol. 1777, Issue 5, pp. 425-32, (2008) (PubMed).
Yoshida, Watanabe, Kato, Sakamoto, Noguchi: "Influence of chloroplastic photo-oxidative stress on mitochondrial alternative oxidase capacity and respiratory properties: a case study with Arabidopsis yellow variegated 2." in: Plant & cell physiology, Vol. 49, Issue 4, pp. 592-603, (2008) (PubMed).
Target
Lhcb1 (lhcb1)
(Chlorophyll a-b binding protein (lhcb1))
AGI Code: At1g29910 The major light-harvesting antenna complex II (LHCII) in photosynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. Lhcb1 is the most abundant chlorophyll a/b-binding protein in eukaryotic phototrophs and often is coded by several nuclear genes.A molecular characterisation of the LHCII proteins can be found in Caffarri et al. (2004) A Look within LHCII: Differential Analysis of the Lhcb1-3 Complexes Building the Major Trimeric Antenna Complex of Higher-Plant Photosynthesis. Biochemistry 43 (29): 9467-9476
Molekulargewicht
expected: 25 kDa, apparent: 25 kDa for Arabidopsis thaliana