Peptide target used to elicit this antibody has a weak, sporadic conservation withGlutamine Synthetase to III, antibody not expected to detect this enzyme. Weak conservation with some Glutaminyl-tRNA synthetase (Glutamine--tRNA ligase)(GLNRS), but antibody not expected to detect this enzyme.
Produktmerkmale
Expected / apparent Molecular Weight of the Antigene: 26 / 25-26 kDa for Arabidopsis thaliana
Aufreinigung
affinity purified
Immunogen
BSA-conjugated synthetic peptide derived from Arabidosis thaliana Lhcb5 protein UniProt: Q9XF89, TAIR: At4g10340 sequence. This sequence is highly conserved in Lhcb5 proteins from monocots, dicots and conifers but only partial conserved in Physcomitrella patens and Chlamydomonas reinhardtii.
Isotyp
IgG
Applikationshinweise
Recommended Dilution: 1 : 1000, detected with standard ECL (WB).
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
For reconstitution add 200 µL of sterile water
Buffer
PBS pH 7.4
Handhabung
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Lagerung
-20 °C
Zienkiewicz, Ferenc, Wasilewska, Romanowska: "High light stimulates Deg1-dependent cleavage of the minor LHCII antenna proteins CP26 and CP29 and the PsbS protein in Arabidopsis thaliana." in: Planta, Vol. 235, Issue 2, pp. 279-88, (2012) (PubMed).
Jung, Lee, Lee, Kang, Kim, Grimm, Back: "Toxic tetrapyrrole accumulation in protoporphyrinogen IX oxidase-overexpressing transgenic rice plants." in: Plant molecular biology, Vol. 67, Issue 5, pp. 535-46, (2008) (PubMed).
Target
LHCB5
(Chlorophyll A-B Binding Protein CP26 (LHCB5))
Andere Bezeichnung
CP26 chlorophyll a/b-binding protein of plant PSII (Lhcb5)
Synonyme
CHLOROPHYLL PROTEIN 26 antikoerper, CP 26 antikoerper, CP26 antikoerper, F24G24.140 antikoerper, F24G24_140 antikoerper, light harvesting complex of photosystem II 5 antikoerper, light harvesting complex of photosystem II 5 antikoerper, LHCB5 antikoerper
Hintergrund
AGI Code: At4g10340 Lhcb5 is one of the 3 minor highly conserved chlorophyll a/b-binding proteins associated with Photosystem II in plants and algae. As a part of the inner light-harvesting antenna it has been sugested to regulate (together with Lhcb4 and Lhcb6) the energy flow from the outer LHCII antenna to the PSII reaction center.
Molekulargewicht
expected: 26 kDa, apparent: 25-26 kDa for Arabidopsis thaliana