RBCL (Isoform 1) Antikörper

Produktnummer ABIN93524

Produktdetails

Target: RBCL

Bindungsspezifität: Isoform 1

Reaktivität: Arabidopsis thaliana, Lobaria pulmonaria, Pisum sativum, Potato, Spinach

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunolocalization (IL)

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 kDa (Chlamydomonas reinhardtii)

Aufreinigung: affinity purified

Immunogen: KLH-conjugated synthetic peptide derived from all known plant,algal and cyanobacterial RbcL (Rubisco large subunit of Rubisco Form I) sequences, including Arabidopsis thaliana UniProt: O03042, TAIR: AtCg00490, Synechococcus sp. Q3ALL1

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: 1 : 10 000 - 1 : 20 000 on 2 µg of total cellular protein, detected with standard ECL(WB), (IL) tested on a grass species, formaldehyde-fixed and paraffin-embeddedtissue following the protocol from Gonzalez et al. (1998) Plant Physiol. V. 116.

Kommentare:

This antibody detects RbcL protein from 102.6 fmoles and has been used as a control to ensure adequate permeabilization and fixation of toxic cyanobacterial cells in immunolabeling experiments (method based on: Orellana & Perry (1995) J Phycol 31: 785-794).Antibody has been used in immunolabelling of intact cyanobacterial cells fixed with ethanol using a secondary anti-IgY antibody conjugated with a fluorochrome.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: For reconstitution add 225 µL of milliQ water per each tube

Konzentration: 16 µg/µL

Buffer: PBS pH 8.0+ 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Make aliquots to avoid working with a stock.
Please, Remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubek.

Lagerung: 4 °C

Referenzen

Abdel-Ghany, Pilon: "MicroRNA-mediated systemic down-regulation of copper protein expression in response to low copper availability in Arabidopsis." in: The Journal of biological chemistry, Vol. 283, Issue 23, pp. 15932-45, (2008) (PubMed).

Ensminger, Schmidt, Lloyd: "Soil temperature and intermittent frost modulate the rate of recovery of photosynthesis in Scots pine under simulated spring conditions." in: The New phytologist, Vol. 177, Issue 2, pp. 428-42, (2008) (PubMed).

Menon, Dou, Heinhorst, Shively, Cannon: "Halothiobacillus neapolitanus carboxysomes sequester heterologous and chimeric RubisCO species." in: PLoS ONE, Vol. 3, Issue 10, pp. e3570, (2008) (PubMed).

Antigendetails

Target: RBCL

Hintergrund: AGI Code: ATCG00490
Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthesis. It is one of the most abundant proteins on Earth and its homology has been demonstrated from purple bacteria to flowering plants.

Molekulargewicht: 52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 kDa (Chlamydomonas reinhardtii)

UniProt: Q3ALL1, O03042