No cross-reactivity with: diatoms, eukaryotic GlnA
Produktmerkmale
Expected / apparent Molecular Weight of the Antigene: 53 kDa
Aufreinigung
affinity purified
Immunogen
KLH-conjugated synthetic peptide derived from available bacterial GlnA sequences with perfect conservation in alpha, beta, gamma Proteobacteria, Enterobacteria, Thermotogales, Low GC Gram+, Cyanobacteria (except weak conservation with Trichodesmium thiebautii) including Synechocystis PCC 6803 Q59981
Isotyp
IgY
Applikationshinweise
Recommended Dilution: 1 : 5000 with standard ECL (WB).
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
16 µg/µL
Buffer
PBS pH 8.0+ 0.02 % sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes. Make aliquots to avoid repreated freeze-thaw cycles and working with a stock.
Lagerung
4 °C
Target
GlnA
Hintergrund
Glutamine synthetase (EC=6.3.1.2) is the key enzyme in the incorporation of mineral nitrogen into glutamine. Activity of this enzyme is controlled by adenylarion under conditions of abundant glutamine.