Aktivieren Sie Javascript in Ihrem Browser, um unsere Webseite optimal zu nutzen.

CD25 Antikörper

Name: CD25 Antikörper
Brand: antibodies-online
SKU: ABIN94044
Price: 273.24 EUR
Availability: InStock

IL2RA Reaktivität: Human FACS Wirt: Maus Monoclonal MEM-181 unconjugated

Flow cytometry surface staining pattern of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD25 (MEM-181) purified antibody (concentration in sample 2 μg/mL) GAM APC. (CD25 Antikörper)

FACS

Surface staining of human PBMC with anti-human CD25 (MEM-181) FITC. (CD25 Antikörper)

FACS

Separation of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD25 (MEM-181) purified antibody (concentration in sample 2 μg/mL, GAM APC, red-filled) from human PHA stimulated peripheral blood mononuclear cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining). (CD25 Antikörper)

FACS

3 Abbildungen

(1 reference)

Produktnummer ABIN94044

Datenblatt (PDF)

Target Alle CD25 (IL2RA) Antikörper anzeigen

CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
Reaktivität
191

130

35

9

6

3

2

2

2

1

1

1
Human
Wirt
150

97

85

2

1

1
Maus
Passende Sekundärantikörper
Klonalität
253

79

2
Monoklonal
Konjugat
113

35

30

28

24

16

8

8

5

5

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser CD25 Antikörper ist unkonjugiert
Applikation
228

91

59

52

44

43

22

17

14

14

10

9

8

7

6

4

3

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1
Flow Cytometry (FACS)

Spezifität

The antibody MEM-181 reacts with an extracellular epitope of CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells), it is lost on resting B and T lymphocytes.

Kreuzreaktivität (Details)

Human

Aufreinigung

Purified by protein-A affinity chromatography.

Reinheit

> 95 % (by SDS-PAGE)

Immunogen

PHA-activated peripheral blood leucocytes

Klon

MEM-181

Isotyp

IgG1

Top Product

Discover our top product IL2RA Primärantikörper
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 201-268) antibody
IL2RA Reaktivität: Human, Maus FACS, WB, ELISA, IHC (p) Wirt: Kaninchen Polyclonal unconjugated

anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody
IL2RA Reaktivität: Human WB, IHC, ELISA, ICC Wirt: Maus Monoclonal 1B5D12 unconjugated

anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 22-213) antibody
IL2RA Reaktivität: Human WB, ELISA, ICC, IHC (fro), IHC (p) Wirt: Kaninchen Polyclonal unconjugated

anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody
IL2RA Reaktivität: Human, Maus, Ratte, Affe WB, IHC, ELISA, IF/ICC Wirt: Maus Monoclonal unconjugated

anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 22-245) antibody
IL2RA Reaktivität: Schwein WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (PerCP)
IL2RA Reaktivität: Human FACS Wirt: Maus Monoclonal MEM-181 PerCP

anti-Interleukin 2 Receptor, alpha (IL2RA) antibody
IL2RA Reaktivität: Maus, Ratte WB, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 42-183) antibody
IL2RA Reaktivität: Human IHC, StM Wirt: Maus Monoclonal IL2RA-2395 unconjugated

anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 22-235) antibody
IL2RA Reaktivität: Maus WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 22-185) antibody
IL2RA Reaktivität: Human WB, IHC, ELISA, IF Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

Flow cytometry: Recommended dilution: 1-5 μg/mL.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Konzentration

1 mg/mL

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung

Do not freeze.

Lagerung

4 °C

Informationen zur Lagerung

Store at 2-8°C. Do not freeze.
Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, (2007) (PubMed).
Target

CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Andere Bezeichnung

CD25 (IL2RA Produkte)

Synonyme

CD25 antikoerper, IDDM10 antikoerper, IL2R antikoerper, TCGFR antikoerper, IL2RAC antikoerper, IL-2RA antikoerper, Il2r antikoerper, Ly-43 antikoerper, interleukin 2 receptor subunit alpha antikoerper, interleukin-2 receptor subunit alpha-like antikoerper, interleukin 2 receptor, alpha chain antikoerper, IL2RA antikoerper, Il2ra antikoerper, LOC100222957 antikoerper

Hintergrund

Interleukin 2 receptor subunit alpha,CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 Molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. Humanized anti CD25 antibodies represent a useful tool to reduce the incidence of allograft rejection as well as the severity of graft versus host reaction, and radioimmunoconjugates of anti-CD25 antibodies can be used against CD25 expressing lymphomas.,IL-2R α, chain, IL-2-RA, IL2-RA, p55, TAC antigen, IDDM10, TCGFR

Gen-ID

3559

UniProt

P01589

Pathways

JAK-STAT Signalweg, Growth Factor Binding, Activated T Cell Proliferation