ITGB1 Antikörper

Produktnummer ABIN94054

Produktdetails anti-ITGB1 Antikörper

Target: ITGB1 (Integrin beta 1 (ITGB1))

Reaktivität: Human, Schwein, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ITGB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Spezifität: The antibody MEM-101A reacts with an extracellular epitope of CD29 (integrin beta1 chain), a 130 kDa single chain type I glycoprotein expressed as a heterodimer (non-covalently associated with the integrin alpha subunits 1-6). CD29 is broadly expressed on majority of hematopoietic and non-hematopoietic cells (leukocytes, platelets, fibroblasts, endothelial cells, epithelial cells and mast cells).

Keine Kreuzreaktivität: Maus

Kreuzreaktivität (Details): Human, Porcine, Canine (Dog)

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Raji Burkitt's lymphoma cell line

Klon: MEM-101A

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blotting: Recommended dilution: 2 μg/mL, positive control: JURKAT human leukemia T-cell lysate, Kg-1a human leukemia cell lysate, non-reducing conditions, 6 % separating gel, 4 % stacking gel.
Flow cytometry: Recommended dilution: 2 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-ITGB1 Antikörper (ABIN94054)

Plánka, Necas, Srnec, Rauser, Starý, Jancár, Amler, Filová, Hlucilová, Kren, Gál: "Use of allogenic stem cells for the prevention of bone bridge formation in miniature pigs." in: Physiological research / Academia Scientiarum Bohemoslovaca, Vol. 58, Issue 6, pp. 885-93, (2010) (PubMed).

Símová, Klíma, Cermak, Sourková, Andera: "Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane." in: Apoptosis : an international journal on programmed cell death, Vol. 13, Issue 3, pp. 423-36, (2008) (PubMed).

Details zu ITGB1

Target: ITGB1 (Integrin beta 1 (ITGB1))

Andere Bezeichnung: CD29 (ITGB1 Produkte)

Synonyme: CD29 antikoerper, FNRB antikoerper, GPIIA antikoerper, MDF2 antikoerper, MSK12 antikoerper, VLA-BETA antikoerper, VLAB antikoerper, 4633401G24Rik antikoerper, AA409975 antikoerper, AA960159 antikoerper, ENSMUSG00000051907 antikoerper, Fnrb antikoerper, Gm9863 antikoerper, gpIIa antikoerper, cd29 antikoerper, fnrb antikoerper, gpiia antikoerper, itgb1 antikoerper, mdf2 antikoerper, msk12 antikoerper, vla-beta antikoerper, vlab antikoerper, itgb1-a antikoerper, itgb1-b antikoerper, integrin subunit beta 1 antikoerper, integrin beta 1 (fibronectin receptor beta) antikoerper, integrin subunit beta 1 S homeolog antikoerper, integrin beta 1 antikoerper, integrin subunit beta 1 L homeolog antikoerper, ITGB1 antikoerper, Itgb1 antikoerper, itgb1.S antikoerper, LOC100009173 antikoerper, itgb1.L antikoerper

Hintergrund: Integrin subunit beta 1,CD29 (beta1 integrin subunit, GPIIa) forms non-covalently linked heterodimers with at least 6 different alpha chains (alpha1-alpha6, CD49a-f) determining the binding properties of beta1 (VLA) integrins. These integrins mediate cell adhesion to collagen, fibronectin, laminin and other extracellular matrix (ECM) components. This interaction hinders cell death, whereas disruption of anchorage to ECM leads to apoptosis. Decreased expression of most beta1 integrins correlates with acquiring multidrug resistance of tumour cells during selection in presence of antitumour drug. In platelets, translocation of intracellular pool of beta1 integrins to the plasma membrane following thrombin stimulation. These integrins are also up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue, as well as in regulating tissue damage and disease symptoms related to inflammatory bowel disease. Through a beta1 integrin-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1beta.,Integrin β,1 chain, ITGB1, VLAB, MDF2, FNRB, GPIIA, MSK12

Gen-ID: 3688

UniProt: P05556

Pathways: Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interaktom