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CD34 Antikörper

Name: CD34 Antikörper
Brand: antibodies-online
SKU: ABIN94077
Price: 286.9 EUR
Availability: InStock

CD34 Reaktivität: Human FACS, WB, IHC (p), ICC Wirt: Maus Monoclonal 4H11[APG] unconjugated

Flow Cytometry analysis Surface staining of Kg-1a human acute myelogenous leukemia cell line with anti-human CD34 (4H11[APG]) FITC. (CD34 Antikörper)

FACS

Western bloting analysis of human CD34 using mouse monoclonal antibody 4H11[APG] on lysates of TF-1 cell line and HEK293T/17 cell line (CD34 non-expressing cell line, negative control) under non-reducing and reducing conditions. (CD34 Antikörper)

Detection of CD34 in human chronic myeloid leukemia cell line MOLM-7 with anti-human CD34 PE. (CD34 Antikörper)

Surface staining of CD34+ cells in human peripheral blood with anti-CD34 (4H11[APG]) purified / GAM-APC. (CD34 Antikörper)

FACS

4 Abbildungen

(1 reference)

Produktnummer ABIN94077

Datenblatt (PDF)

Target Alle CD34 Antikörper anzeigen

CD34
Reaktivität
301

116

107

33

19

17

17

17

17

17

9

6

6

1

1
Human
Wirt
170

144

22

6

4
Maus
Passende Sekundärantikörper
Klonalität
224

119

2
Monoklonal
Konjugat
123

28

28

23

18

8

6

6

5

5

5

5

5

4

4

4

4

4

4

4

4

4

4

4

3

3

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1
Dieser CD34 Antikörper ist unkonjugiert
Applikation
229

196

88

68

62

47

39

37

34

28

23

16

13

7

7

6

4

4

4

4

3

3

2

2

1

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Spezifität

The mouse monoclonal antibody 4H11[APG] reacts with extracellular class III epitope on CD34, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells, it is gradually lost on progenitor cells. The antibody 4H11[APG] completely blocks binding of class III antibodies BIRMA K3 and 8G12 on KG1a cell line.

Kreuzreaktivität (Details)

Human

Aufreinigung

Purified by protein-A affinity chromatography.

Reinheit

> 95 % (by SDS-PAGE)

Immunogen

Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia.

Klon

4H11[APG]

Isotyp

IgG1

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anti-CD34 (CD34) (AA 301-385) antibody
CD34 Reaktivität: Human, Maus, Ratte, Kaninchen, Ziege, Huhn FACS, WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) Wirt: Kaninchen Polyclonal unconjugated

anti-CD34 (CD34) antibody
CD34 Reaktivität: Human, Non-Human Primate FACS, WB, IHC (p), IP, IHC (fro), Func Wirt: Maus Monoclonal QBEnd-10 unconjugated

anti-CD34 (CD34) (AA 36-287) antibody
CD34 Reaktivität: Maus WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-CD34 (CD34) antibody (PE)
CD34 Reaktivität: Human FACS, ICC Wirt: Maus Monoclonal 4H11[APG] PE

anti-CD34 (CD34) antibody
CD34 Reaktivität: Human, Ratte FACS, IHC, StM Wirt: Kaninchen Monoclonal HPCA1-2598R unconjugated Recombinant Antibody

anti-CD34 (CD34) (C-Term) antibody
CD34 Reaktivität: Human WB, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-CD34 (CD34) (AA 151-385) antibody
CD34 Reaktivität: Human, Maus, Ratte FACS, WB, IHC (p) Wirt: Kaninchen Polyclonal unconjugated

anti-CD34 (CD34) (AA 124-375) antibody
CD34 Reaktivität: Human WB, IHC, IF, ELISA Wirt: Kaninchen Polyclonal unconjugated

anti-CD34 (CD34) antibody (FITC)
CD34 Reaktivität: Human, Non-Human Primate FACS Wirt: Maus Monoclonal 581 FITC

Applikationshinweise

Western blotting: Recommended dilution: 1-2 μg/mL, positive control: Kg-1a, TF-1 cells, non-reducing conditions.
Flow cytometry: Recommended dilution: 2 μg/mL.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: placenta endothelium.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Konzentration

1 mg/mL

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung

Do not freeze.

Lagerung

4 °C

Informationen zur Lagerung

Store at 2-8°C. Do not freeze.
Elknerová, Lacinová, Soucek, Marinov, Stöckbauer: "Growth inhibitory effect of the antibody to hematopoietic stem cell antigen CD34 in leukemic cell lines." in: Neoplasma, Vol. 54, Issue 4, pp. 311-20, (2007) (PubMed).
Target

CD34

Andere Bezeichnung

CD34 (CD34 Produkte)

Synonyme

AU040960 antikoerper, CD34 molecule antikoerper, CD34 antigen antikoerper, CD34 antikoerper, Cd34 antikoerper

Hintergrund

CD34 Molecule,CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.

Gen-ID

947

UniProt

P28906