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CD34 Antikörper (PE)

Name: CD34 Antikörper (PE)
Brand: antibodies-online
SKU: ABIN94080
Price: 430.35 EUR
Availability: InStock

CD34 Reaktivität: Human FACS, ICC Wirt: Maus Monoclonal 4H11[APG] PE

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD34 (4H11[APG]) PE antibody (20 μL reagent / 100 μL of peripheral whole blood). (CD34 Antikörper (PE))

FACS

Flow Cytometry analysis Surface staining of Kg-1a human acute myelogenous leukemia cell line with anti-human CD34 (4H11[APG]) FITC. (CD34 Antikörper (PE))

FACS

Detection of CD34 in human chronic myeloid leukemia cell line MOLM-7 with anti-human CD34 PE. (CD34 Antikörper (PE))

Immunocytochemistry detection of CD34 in human chronic myeloid leukemia cell line MOLM-7 with anti-human CD34 (4H11[APG]) PE. (CD34 Antikörper (PE))

ICC

Separation of human CD34 positive CD45dim stem cells (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD34 (4H11[APG]) PE antibody (20 μL reagent / 100 μL of peripheral whole blood). (CD34 Antikörper (PE))

FACS

5 Abbildungen

(1 reference)

Produktnummer ABIN94080

Datenblatt (PDF)

Target Alle CD34 Antikörper anzeigen

CD34
Reaktivität
301

116

107

33

19

17

17

17

17

17

9

6

6

1

1
Human
Wirt
170

144

22

6

4
Maus
Passende Sekundärantikörper
Klonalität
224

119

2
Monoklonal
Konjugat
124

28

28

22

18

8

6

6

5

5

5

5

5

4

4

4

4

4

4

4

4

4

4

4

3

3

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1
Dieser CD34 Antikörper ist konjugiert mit PE
Applikation
229

197

88

68

62

48

39

37

34

28

23

16

13

7

7

6

4

4

4

4

3

3

2

2

1

1

1

1

1

1
Flow Cytometry (FACS), Immunocytochemistry (ICC)

Spezifität

The mouse monoclonal antibody 4H11[APG] reacts with extracellular class III epitope on CD34, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells, it is gradually lost on progenitor cells. The antibody 4H11[APG] completely blocks binding of class III antibodies BIRMA K3 and 8G12 on KG1a cell line.

Kreuzreaktivität (Details)

Human

Aufreinigung

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen

Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia.

Klon

4H11[APG]

Isotyp

IgG1

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anti-CD34 (CD34) (AA 301-385) antibody
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CD34 Reaktivität: Maus WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated

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CD34 Reaktivität: Human, Ratte FACS, IHC, StM Wirt: Kaninchen Monoclonal HPCA1-2598R unconjugated Recombinant Antibody

anti-CD34 (CD34) (C-Term) antibody
CD34 Reaktivität: Human WB, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-CD34 (CD34) (AA 151-385) antibody
CD34 Reaktivität: Human, Maus, Ratte FACS, WB, IHC (p) Wirt: Kaninchen Polyclonal unconjugated

anti-CD34 (CD34) antibody
CD34 Reaktivität: Human FACS, WB, IHC (p), ICC Wirt: Maus Monoclonal 4H11[APG] unconjugated

anti-CD34 (CD34) (AA 124-375) antibody
CD34 Reaktivität: Human WB, IHC, IF, ELISA Wirt: Kaninchen Polyclonal unconjugated

anti-CD34 (CD34) antibody (FITC)
CD34 Reaktivität: Human, Non-Human Primate FACS Wirt: Maus Monoclonal 581 FITC

Applikationshinweise

Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Kommentare

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Rekonstitution

No reconstitution is necessary.

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung

Do not freeze.
Avoid prolonged exposure to light.

Lagerung

4 °C

Informationen zur Lagerung

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Elknerová, Lacinová, Soucek, Marinov, Stöckbauer: "Growth inhibitory effect of the antibody to hematopoietic stem cell antigen CD34 in leukemic cell lines." in: Neoplasma, Vol. 54, Issue 4, pp. 311-20, (2007) (PubMed).
Target

CD34

Andere Bezeichnung

CD34 (CD34 Produkte)

Synonyme

AU040960 antikoerper, CD34 molecule antikoerper, CD34 antigen antikoerper, CD34 antikoerper, Cd34 antikoerper

Hintergrund

CD34 Molecule,CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.

Gen-ID

947

UniProt

P28906