CD4 Antikörper (N-Term)

Produktnummer ABIN94088

Produktdetails anti-CD4 Antikörper

Target: CD4

Bindungsspezifität: N-Term

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD4 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Cytometry by Time of Flight (CyTOF)

Spezifität: The antibody MEM-241 recognizes an extracellular epitope of CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes (“,helper“, T-cells) and also on monocytes, tissue macrophages and granulocytes.

Kreuzreaktivität (Details): Human, Other not tested

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: 2 N-terminal domains of human CD4 fused to human IgG1 Fc

Klon: MEM-241

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Recommended dilution: 1-5 μg/mL, positive control: peripheral blood, HPB T cell line. This antibody is excellent antibody for FC application.
Western blotting: Recommended dilution: 1-2 μg/mL, positive control: peripheral blood, HPB T cell line, non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-CD4 Antikörper (ABIN94088)

Holmannova, Kolackova, Mandak, Kunes, Holubcova, Krejsek, Vlaskova, Andrys: "Inhibitory CD200R and proapoptotic CD95/CD95L molecules on innate immunity cells are modulated by cardiac surgery." in: Perfusion, (2014) (PubMed).

Hovden, Karlsen, Jonsson, Aarstad, Appel: "Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses." in: BMC immunology, Vol. 12, pp. 2, (2011) (PubMed).

Anderson, Sayers, Haniffa, Swan, Diboll, Wang, Isaacs, Hilkens: "Differential regulation of naïve and memory CD4+ T cells by alternatively activated dendritic cells." in: Journal of leukocyte biology, Vol. 84, Issue 1, pp. 124-33, (2008) (PubMed).

Karlsson, Cowley, Martinez, Shaw, Minger, James: "Homogeneous monocytes and macrophages from human embryonic stem cells following coculture-free differentiation in M-CSF and IL-3." in: Experimental hematology, Vol. 36, Issue 9, pp. 1167-75, (2008) (PubMed).

Manasa, Musabaike, Masimirembwa, Burke, Luthy, Mudzori: "Evaluation of the Partec flow cytometer against the BD FACSCalibur system for monitoring immune responses of human immunodeficiency virus-infected patients in Zimbabwe." in: Clinical and vaccine immunology : CVI, Vol. 14, Issue 3, pp. 293-8, (2007) (PubMed).

Zola, Swart, Banham, Barry, Beare, Bensussan, Boumsell, D Buckley, Bühring, Clark, Engel, Fox, Jin, Macardle, Malavasi, Mason, Stockinger, Yang: "CD molecules 2006--human cell differentiation molecules." in: Journal of immunological methods, Vol. 319, Issue 1-2, pp. 1-5, (2007) (PubMed).

Brdicková, Brdicka, Angelisová, Horváth, Spicka, Hilgert, Paces, Simeoni, Kliche, Merten, Schraven, Horejsí: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, (2003) (PubMed).

Details zu CD4

Target: CD4

Andere Bezeichnung: CD4 (CD4 Produkte)

Synonyme: cd4 antikoerper, L3T4 antikoerper, Ly-4 antikoerper, CD4mut antikoerper, W3/25 antikoerper, p55 antikoerper, CD4 molecule antikoerper, carcinoembryonic antigen-related cell adhesion molecule 5 antikoerper, T-cell surface glycoprotein CD4 antikoerper, CD4 antigen antikoerper, Cd4 molecule antikoerper, CD4 antikoerper, LOC100125525 antikoerper, cd4 antikoerper, Cd4 antikoerper

Hintergrund: CD4 Molecule,CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1), IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus, CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).,T4/Leu-3, L3T4

Gen-ID: 920

UniProt: P01730

Pathways: T-Zell Rezeptor Signalweg, Maintenance of Protein Location, CXCR4-mediated Signaling Events