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CD43 Antikörper

SPN Reaktivität: Human FACS, WB, IHC (p), IP Wirt: Maus Monoclonal MEM-59 unconjugated
Produktnummer ABIN94116
  • Target Alle CD43 (SPN) Antikörper anzeigen
    CD43 (SPN) (Sialophorin (SPN))
    Reaktivität
    • 150
    • 33
    • 8
    Human
    Wirt
    • 95
    • 64
    • 27
    Maus
    Klonalität
    • 101
    • 84
    • 1
    Monoklonal
    Konjugat
    • 65
    • 21
    • 19
    • 19
    • 14
    • 10
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser CD43 Antikörper ist unkonjugiert
    Applikation
    • 103
    • 96
    • 62
    • 62
    • 42
    • 18
    • 17
    • 12
    • 6
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
    Spezifität
    The antibody MEM-59 recognizes a neuraminidase-sensitive extracellular epitope on CD43 (Leukosialin), a 95-135 kDa type I transmembrane glycoprotein (mucin-type) which is involved in lymphocyte activation. CD43 is expressed by platelets and at high levels on the surface of all leukocytes, it is negative on resting B lymphocytes and erythrocytes.
    Kreuzreaktivität (Details)
    Human
    Aufreinigung
    Purified by protein-A affinity chromatography.
    Reinheit
    > 95 % (by SDS-PAGE)
    Immunogen
    Human T lymphocytes.
    Klon
    MEM-59
    Isotyp
    IgG1
  • Applikationshinweise
    Flow cytometry: Recommended dilution: 1-5 μg/mL.
    Immunohistochemistry (paraffin sections): Positive tissue: spleen, thymus, tonsil, recommended dilution: 10 μg/mL.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Do not freeze.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store at 2-8°C. Do not freeze.
  • Schatzlmaier, Supper, Göschl, Zwirzitz, Eckerstorfer, Ellmeier, Huppa, Stockinger: "Rapid multiplex analysis of lipid raft components with single-cell resolution." in: Science signaling, Vol. 8, Issue 395, pp. rs11, (2015) (PubMed).

    Símová, Klíma, Cermak, Sourková, Andera: "Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane." in: Apoptosis : an international journal on programmed cell death, Vol. 13, Issue 3, pp. 423-36, (2008) (PubMed).

    Filatov, Krotov, Zgoda, Volkov: "Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass-spectrometry." in: Journal of immunological methods, Vol. 319, Issue 1-2, pp. 21-33, (2007) (PubMed).

    Cermák, Símová, Pintzas, Horejsí, Andera: "Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules." in: The Journal of biological chemistry, Vol. 277, Issue 10, pp. 7955-61, (2002) (PubMed).

    Alvarado, Klassen, Cerny, Horejsí, Schmidt: "MEM-59 monoclonal antibody detects a CD43 epitope involved in lymphocyte activation." in: European journal of immunology, Vol. 25, Issue 4, pp. 1051-5, (1995) (PubMed).

    Stefanová, Hilgert, Angelisová, Kristofová, Horejsí: "Characterization of a 95 kDa human leucocyte sialoglycoprotein: its identity with CD43, gpL115, leukosialin and sialophorin." in: Folia biologica, Vol. 34, Issue 4, pp. 255-65, (1989) (PubMed).

  • Target
    CD43 (SPN) (Sialophorin (SPN))
    Andere Bezeichnung
    CD43 (SPN Produkte)
    Synonyme
    CD43 antikoerper, GALGP antikoerper, GPL115 antikoerper, LSN antikoerper, A630014B01Rik antikoerper, Cd43 antikoerper, Galgp antikoerper, Ly-48 antikoerper, Ly48 antikoerper, Lsn antikoerper, Lsn1 antikoerper, gpL115 antikoerper, sialophorin antikoerper, SPN antikoerper, Spn antikoerper
    Hintergrund
    Sialophorin,CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumour suppressor p53 protein, but in case of p53 and ARF defficiency CD43 promotes tumour proliferation and viability. It appears to be an important modulator of leukocyte functions.,Leukosialin, Sialophorin, Galactoglycoprotein, GALGP, LSN, SPN, GALGP, GP5
    Gen-ID
    6693
    UniProt
    P16150
    Pathways
    Regulation of Leukocyte Mediated Immunity
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