CD5 Antikörper

Produktnummer ABIN94161

Produktdetails anti-CD5 Antikörper

Target: CD5

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD5 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: The antibody MEM-32 reacts with an extracellular epitope of CD5, a 67 kDa single-chain transmembrane glycoprotein expressed on mature T-lymphocytes, most of thymocytes and B-lymphocytes subset (B-1a lymphocytes).

Kreuzreaktivität (Details): Human

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Crude thymus membrane fraction.

Klon: MEM-32

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA: The antibody MEM-32 can be used in the Sandwich ELISA as the capture antibody in pair with the detection antibody CRIS1.
Immunohistochemistry (paraffin sections): Recommended dilution: 20 μg/mL, positive tissue: spleen.
Flow cytometry: Recommended dilution: 2 μg/mL.
Western blotting: Laurylmaltoside lysing buffer, non-reducing conditions, recommended dilution: 1-2 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-CD5 Antikörper (ABIN94161)

Hrdinka, Dráber, Stepánek, Ormsby, Otáhal, Angelisová, Brdicka, Paces, Horejsí, Drbal: "PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis." in: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 19617-29, (2011) (PubMed).

Details zu CD5

Target: CD5

Andere Bezeichnung: CD5 (CD5 Produkte)

Synonyme: CD5 antikoerper, Ly-1 antikoerper, Ly-12 antikoerper, Ly-A antikoerper, Lyt-1 antikoerper, LEU1 antikoerper, T1 antikoerper, CD5 molecule antikoerper, CD5 antigen antikoerper, CD5 molecule like antikoerper, Cd5 molecule antikoerper, CD5 antikoerper, Cd5 antikoerper, CD5L antikoerper

Hintergrund: CD5 Molecule,CD5 antigen (T1, 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains. The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells it seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies. Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (rheumatoid arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.,T1, LEU1

Gen-ID: 921

UniProt: P06127