CD59 Antikörper

Produktnummer ABIN94203

Produktdetails anti-CD59 Antikörper

Target: CD59

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD59 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: The antibody MEM-43/5 reacts with well defined epitope (around L33) on CD59 (Protectin), a 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored extracellular glycoprotein expressed on the surface of all hematopoietic cells, it is widely present on cells in all tissues. The MEM-43/5 does not compete with most other CD59 antibodies.

Kreuzreaktivität (Details): Mouse, Human

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Thymocytes and T lymphocytes

Klon: MEM-43-5

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Recommended dilution: 0.5-4 μg/mL, positive control: blood.
Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/mL.
Western blotting: Recommended dilution: 1-2 μg/mL, positive control: mouse spleen, non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-CD59 Antikörper (ABIN94203)

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, (2007) (PubMed).

Stulnig, Berger, Sigmund, Stockinger, Horejsí, Waldhäusl: "Signal transduction via glycosyl phosphatidylinositol-anchored proteins in T cells is inhibited by lowering cellular cholesterol." in: The Journal of biological chemistry, Vol. 272, Issue 31, pp. 19242-7, (1997) (PubMed).

Bodian, Davis, Morgan, Rushmere: "Mutational analysis of the active site and antibody epitopes of the complement-inhibitory glycoprotein, CD59." in: The Journal of experimental medicine, Vol. 185, Issue 3, pp. 507-16, (1997) (PubMed).

Details zu CD59

Target: CD59

Andere Bezeichnung: CD59 (CD59 Produkte)

Synonyme: 16.3A5 antikoerper, 1F5 antikoerper, EJ16 antikoerper, EJ30 antikoerper, EL32 antikoerper, G344 antikoerper, HRF-20 antikoerper, HRF20 antikoerper, MAC-IP antikoerper, MACIF antikoerper, MEM43 antikoerper, MIC11 antikoerper, MIN1 antikoerper, MIN2 antikoerper, MIN3 antikoerper, MIRL antikoerper, MSK21 antikoerper, p18-20 antikoerper, Cd59a antikoerper, Cd59b antikoerper, MACIP antikoerper, CD59 molecule (CD59 blood group) antikoerper, CD59 molecule antikoerper, CD59 antikoerper, Cd59 antikoerper

Hintergrund: CD59 Molecule,CD59 (protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.,MACIF, MAC-IP, protectin, MIRL, HRF-20, HRF20, 1F5, MIN

Gen-ID: 966

UniProt: P13987

Pathways: Komplementsystem