pan Keratin Antikörper

Produktnummer ABIN94275

Produktdetails anti-pan Keratin Antikörper

Target: pan Keratin (panKRT)

Reaktivität: Säugetier

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser pan Keratin Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Spezifität: The antibody C-11 reacts with cytokeratin peptides 4, 5, 6, 8, 10, 13, 18. Cytokeratins are members of intermediate filaments subfamily intracellular proteins represented in epithelial tissues.

Kreuzreaktivität (Details): Mammalian

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Keratin-enriched preparation from human epidermoid carcinoma cell line A431.

Klon: C-11

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Recommended dilution: 1 μg/mL. Intracellular staining.
Immunohistochemistry: Recommended dilution: 2-8 μg/mL.
Western blotting: Recommended dilution: 1-2 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-pan Keratin Antikörper (ABIN94275)

Haun, Devaraj, Marinelli, Lee, Weissleder: "Probing intracellular biomarkers and mediators of cell activation using nanosensors and bioorthogonal chemistry." in: ACS nano, Vol. 5, Issue 4, pp. 3204-13, (2011) (PubMed).

Bianchi, Bombelli, Raimondo, Torsello, Angeloni, Ferrero, Di Stefano, Chinello, Cifola, Invernizzi, Brambilla, Magni, Pitto, Zanetti, Mocarelli, Perego: "Primary cell cultures from human renal cortex and renal-cell carcinoma evidence a differential expression of two spliced isoforms of Annexin A3." in: The American journal of pathology, Vol. 176, Issue 4, pp. 1660-70, (2010) (PubMed).

Broekema, Harmsen, Koerts, Petersen, van Luyn, Navis, Popa: "Determinants of tubular bone marrow-derived cell engraftment after renal ischemia/reperfusion in rats." in: Kidney international, Vol. 68, Issue 6, pp. 2572-81, (2005) (PubMed).

Hamakawa, Sumida, Tanioka, Sogawa, Yamada: "Extraction of cytokeratin from the human submandibular gland and its electrophoretic analysis." in: Research communications in molecular pathology and pharmacology, Vol. 101, Issue 2, pp. 115-26, (1999) (PubMed).

Bártek, Vojt?sek, Stasková, Bártková, Kerekés, Rejthar, Kovarík: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, (1991) (PubMed).

Kovarík, Rejthar, Lauerová, Vojt?sek, Bártková: "Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread." in: International journal of cancer. Supplement = Journal international du cancer. Supplement, Vol. 3, pp. 50-5, (1989) (PubMed).

Details zu pan Keratin

Target: pan Keratin (panKRT)

Andere Bezeichnung: Cytokeratin (Pan-reactive) (panKRT Produkte)

Synonyme: xk70a antikoerper, keratin antikoerper, keratin S homeolog antikoerper, LOC431321 antikoerper, krt.S antikoerper

Hintergrund: Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.,cytokeratin, CYK, CK, KRT