Cytokeratin 18 Antikörper
-
- Target Alle Cytokeratin 18 (KRT18) Antikörper anzeigen
- Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))
-
Reaktivität
- Human
-
Wirt
- Maus
-
Klonalität
- Monoklonal
-
Konjugat
- Dieser Cytokeratin 18 Antikörper ist unkonjugiert
-
Applikation
- Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Spezifität
- The mouse monoclonal antibody DC-10 reacts with cytokeratin 18 (45 kDa). Cytokeratins are members of intermediate filaments subfamily of intracellular proteins, represented in epithelial tissues.
- Keine Kreuzreaktivität
- Rind (Kuh), Hund, Hamster, Maus, Schwein, Ratte, Schaf
- Kreuzreaktivität (Details)
- Human
- Aufreinigung
- Purified by protein-A affinity chromatography.
- Reinheit
- > 95 % (by SDS-PAGE)
- Immunogen
- Human breast carcinoma cell line PMC-42.
- Klon
- DC-10
- Isotyp
- IgG1
-
-
- Applikationshinweise
-
Western blotting: Recommended dilution: 1-2 μg/mL.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: colon, skin.
Immunocytochemistry: positive control: MCF-7 human breast adenocarcinoma cell line, HeLa human cervix carcinoma cell line.
ELISA: Positive control: MCF-7 human breast adenocarcinoma cell line, the antibody DC-10 has been tested as the detection antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with the antibody C-04 (cat. no. 11-106-C100).
Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Konzentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Do not freeze.
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Store at 2-8°C. Do not freeze.
-
-
Primary human hepatocytes on biodegradable poly(l-lactic acid) matrices: a promising model for improving transplantation efficiency with tissue engineering." in: Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society, Vol. 17, Issue 2, pp. 104-14, (2011) (PubMed).
: "Monoclonal antibodies recognizing different epitopes of cytokeratin No.18." in: Folia biologica, Vol. 35, Issue 6, pp. 373-82, (1990) (PubMed).
: "Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread." in: International journal of cancer. Supplement = Journal international du cancer. Supplement, Vol. 3, pp. 50-5, (1989) (PubMed).
: "Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule." in: Hybridoma, Vol. 7, Issue 5, pp. 495-504, (1989) (PubMed).
: "
-
Primary human hepatocytes on biodegradable poly(l-lactic acid) matrices: a promising model for improving transplantation efficiency with tissue engineering." in: Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society, Vol. 17, Issue 2, pp. 104-14, (2011) (PubMed).
-
- Target
- Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))
- Andere Bezeichnung
- Cytokeratin 18 (KRT18 Produkte)
- Hintergrund
- Keratin 18,Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratin 18 belongs to type I family (acidic cytokeratins).,K18, CK18, CYK18, KRT18
- Gen-ID
- 3875
- UniProt
- P05783
- Pathways
- Apoptose, Caspase Kaskade in der Apoptose
-