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Cytokeratin 19 Antikörper

Name: Cytokeratin 19 Antikörper
Brand: antibodies-online
SKU: ABIN94290
Price: 317.26 EUR
Availability: InStock

KRT19 Reaktivität: Human WB, ELISA, FACS, IHC (p), ICC, IP Wirt: Maus Monoclonal A53-B-A2 unconjugated

Western Blotting analysis (reducing conditions) of whole cell lysate of MCF-7 human breast adenocarcinoma cell line. (Cytokeratin 19 Antikörper)

Flow cytometry intracellular staining pattern of human peripheral whole blood spiked with MCF-7 cells stained using anti-Cytokeratin 19 (A53-B/A2) purified antibody (concentration in sample 3 μg/mL, GAM APC). (Cytokeratin 19 Antikörper)

FACS

Immunohistochemistry staining of human prostate (paraffin sections) using anti-cytokeratin 19 (A53-B/A2). (Cytokeratin 19 Antikörper)

IHC (p)

Immunohistochemistry staining (paraffin sections) of cytokeratin 19 in human liver using mouse monoclonal antibody A53-B/A2 (ABIN94290, diluted 1:100), detected with GAM IgG-Alexa Fluor®488 (diluted 1:200; green), cell nuclei stained with PI (1µg/ml; orange). (Cytokeratin 19 Antikörper)

IHC (p)

Western blotting analysis of human cytokeratin 19 using mouse monoclonal antibody A53-B/A2 on lysates of HT-29 cell line and Jurkat cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. (Cytokeratin 19 Antikörper)

Separation of MCF-7 cells (red-filled) from human leukocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood spiked with MCF-7 cells stained using anti-Cytokeratin 19 (A53-B/A2) purified antibody (concentration in sample 3 μg/mL, GAM APC). (Cytokeratin 19 Antikörper)

FACS

6 Abbildungen

(2 Referenzen)

Produktnummer ABIN94290

Datenblatt (PDF)

Target Alle Cytokeratin 19 (KRT19) Antikörper anzeigen

Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))
Reaktivität
270

95

76

7

7

6

5

5

4

2

2

2

2

2

2

1

1

1

1

1
Human
Wirt
142

135

3

2

1
Maus
Passende Sekundärantikörper
Klonalität
144

139
Monoklonal
Konjugat
124

32

20

13

7

5

4

4

4

4

4

4

4

4

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

2

2

1

1
Dieser Cytokeratin 19 Antikörper ist unkonjugiert
Applikation
192

95

85

81

68

44

41

39

33

30

22

21

7

6

6

5

2

1

1

1

1

1

1
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Spezifität

The mouse monoclonal antibody A53-B/A2 recognizes the rod domain of cytokeratin 19 (40 kDa), an intracellular antigen constituting intermmediate cytoskeleton filaments. Cytokeratin 19 is not expressed in hepatocytes, it is often co-expressed with cytokeratin 7.

Kreuzreaktivität (Details)

Human

Aufreinigung

Purified by protein-A affinity chromatography.

Reinheit

> 95 % (by SDS-PAGE)

Immunogen

MCF-7 human breast adenocarcinoma cell line

Klon

A53-B-A2

Isotyp

IgG2a
anti-Keratin 19 (KRT19) (AA 245-391) antibody
KRT19 Reaktivität: Human WB, IHC, ICC, IP Wirt: Kaninchen Polyclonal unconjugated

anti-Keratin 19 (KRT19) (AA 245-391) antibody
KRT19 Reaktivität: Human WB, IHC, ICC, IP Wirt: Maus Monoclonal 1# unconjugated

anti-Keratin 19 (KRT19) (AA 1-403) antibody
KRT19 Reaktivität: Maus WB, IHC, ICC, IP Wirt: Kaninchen Polyclonal unconjugated

anti-Keratin 19 (KRT19) (AA 127-322) antibody
KRT19 Reaktivität: Human WB, IHC, ICC, IP Wirt: Maus Monoclonal D1 unconjugated

anti-Keratin 19 (KRT19) antibody
KRT19 Reaktivität: Human WB, FACS, IHC (p), ICC, IP Wirt: Maus Monoclonal BA-17 unconjugated

anti-Keratin 19 (KRT19) (AA 274-400) antibody
KRT19 Reaktivität: Human WB, IHC, ELISA, FACS, ICC Wirt: Maus Monoclonal 8H1A8 unconjugated

anti-Keratin 19 (KRT19) (AA 80-400) antibody
KRT19 Reaktivität: Human, Ratte WB, IHC, ELISA, FACS Wirt: Maus Monoclonal 2A1G9 unconjugated

anti-Keratin 19 (KRT19) antibody
KRT19 Reaktivität: Human, Maus, Ratte IHC, FACS, IF, StM Wirt: Maus Monoclonal KRT19-800 unconjugated

anti-Keratin 19 (KRT19) antibody
KRT19 Reaktivität: Human WB, IHC, FACS, IF, StM Wirt: Maus Monoclonal KRT19-799 unconjugated

anti-Keratin 19 (KRT19) (AA 241-400) antibody
KRT19 Reaktivität: Human WB, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

Flow cytometry: Recommended dilution: 1-5 μg/mL. Intracellular staining.
Immunohistochemistry: Recommended dilution: 5-10 μg/mL.
Western blotting: Recommended dilution: 1-2 μg/mL, positive control: MCF-7, BA-17, HT-29 cells.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Konzentration

1 mg/mL

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung

Do not freeze.

Lagerung

4 °C

Informationen zur Lagerung

Store at 2-8°C. Do not freeze.
Kasper, Moll, Stosiek, Karsten: "Patterns of cytokeratin and vimentin expression in the human eye." in: Histochemistry, Vol. 89, Issue 4, pp. 369-77, (1988) (PubMed).

Karsten, Papsdorf, Roloff, Stolley, Abel, Walther, Weiss: "Monoclonal anti-cytokeratin antibody from a hybridoma clone generated by electrofusion." in: European journal of cancer & clinical oncology, Vol. 21, Issue 6, pp. 733-40, (1985) (PubMed).
Target

Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Andere Bezeichnung

Cytokeratin 19 (KRT19 Produkte)

Synonyme

CK19 antikoerper, K19 antikoerper, K1CS antikoerper, AI663979 antikoerper, EndoC antikoerper, Krt-1.19 antikoerper, Krt1-19 antikoerper, Ka19 antikoerper, k19 antikoerper, ck19 antikoerper, k1cs antikoerper, krt9 antikoerper, krt15 antikoerper, MGC76282 antikoerper, GK-19 antikoerper, MGC83069 antikoerper, KRT19 antikoerper, keratin 19 antikoerper, keratin 19 L homeolog antikoerper, keratin, type I cytoskeletal 19 antikoerper, KRT19 antikoerper, Krt19 antikoerper, krt19 antikoerper, krt19.L antikoerper, LOC100344434 antikoerper, LOC101117946 antikoerper

Hintergrund

Keratin 19,Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.,K19, CK19, CYK19, KRT19, K1CS

Gen-ID

3880

UniProt

P08727