NEFH Antikörper

Produktnummer ABIN94433

Produktdetails anti-NEFH Antikörper

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reaktivität: Säugetier

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NEFH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Spezifität: The antibody NF-01 recognizes a phosphorylated epitope on heavy neurofilament protein (210 kDa intracellular antigen) of various species. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.

Kreuzreaktivität (Details): Mammalian

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Pellet of porcine brain cold-stable proteins after depolymerization of microtubules.

Klon: NF-01

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (paraffin sections): Recommended dilution: 5-10 μg/mL.
Western blotting: Recommended dilution: 1-2 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-NEFH Antikörper (ABIN94433)

Lukás, Dráber, Bucek, Dráberová, Viklický, Dolezel: "Expression of phosphorylated high molecular weight neurofilament protein (NF-H) and vimentin in human developing dorsal root ganglia and spinal cord." in: Histochemistry, Vol. 100, Issue 6, pp. 495-502, (1994) (PubMed).

Details zu NEFH

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Andere Bezeichnung: Neurofilament heavy protein (NEFH Produkte)

Synonyme: NF-H antikoerper, NF200 antikoerper, Nfh antikoerper, mKIAA0845 antikoerper, NFH antikoerper, NEFH antikoerper, neurofilament heavy polypeptide antikoerper, neurofilament, heavy polypeptide antikoerper, neurofilament heavy antikoerper, MCYG_06231 antikoerper, Nefh antikoerper, NEFH antikoerper

Hintergrund: Neurofilament heavy,Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs in most vertebrates are composed of three different polypeptide chains with different molecular weights –, neurofilament heavy protein (NF-H), medium (NF-M) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. The protein segment on the C-terminal side of the human NF-H rod is uniquely long (more than 600 amino acids) compared to other IF proteins and is highly charged (> 24 % Glu, > 25 % Lys), rich in proline (> 12 %) and improverished in cysteine, methionine and aromatic amino acids. Its most remarkable feature is a repetitive sequence that covers more than half its lenght and includes the sekvence motif Lys-Ser-Pro (KSP) greater than 40 times. Plasma neurofilament heavy chain level has been proposed as a marker of axonal injury and clinical use of its degeneration and loss has been suggested as a biomarker of several neurodegenerative diseases.,NFH, NEFH, Neurofilament heavy chain, CMT2CC

Gen-ID: 4744

UniProt: P12036