CDK1 Antikörper

Produktnummer ABIN94446

Produktdetails anti-CDK1 Antikörper

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Reaktivität: Human, Rind (Kuh), Non-Human Primate

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CDK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Spezifität: The antibody POH-1 specifically detects Cdk1 (p34Cdc2, an intracellular antigen). Staining of Cdk1 reflects the proliferating potential of respective tissue.

Keine Kreuzreaktivität: Drosophila melanogaster, Maus, Ratte, Xenopus laevis

Kreuzreaktivität (Details): Human, Non-Human Primates, Bovine

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Bacterially expressed full-length human Cdk1 protein

Klon: POH-1

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: thymus.
Western blotting: Recommended dilution: 1-2 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-CDK1 Antikörper (ABIN94446)

Lukás, Draetta, Bartek: "Distinct forms of human CDC2 identified by novel monoclonal antibodies." in: European journal of biochemistry / FEBS, Vol. 207, Issue 1, pp. 169-76, (1992) (PubMed).

Details zu CDK1

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Andere Bezeichnung: Cdk1 (CDK1 Produkte)

Hintergrund: Cyclin dependent kinase 1,Cdk1 (cyclin-dependent kinase 1), also known as p34Cdc2 (cell division control protein kinase 2) depends on cyclin A and B and is triggered by a positive feedback loop at the end of G2 phase, which is the key event that initiates mitotic entry. Destruction of cyclin B during metaphase results in inactivation of Cdk1, allowing mitotic exit and cell division. Cdk1 also contributes to the control of DNA replication. Cdk1 can be ihibited by several transcriptional targets of p53, such as p21WAF.,p34Cdc2, p34 protein kinase, CDK1, CDC2, CDC28A

Gen-ID: 983

UniProt: B7Z3D6

Pathways: Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA