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Phosphotyrosine Antikörper

Name: Phosphotyrosine Antikörper
Brand: antibodies-online
SKU: ABIN94452
Price: 334.72 EUR
Availability: InStock

Reaktivität: Diverse Spezies WB, FACS, ICC Wirt: Maus Monoclonal P-Tyr-01 unconjugated

Anti-Phosphotyrosine purified antibody (clone P-Tyr-01) Specificity Verification by Flow Cytometry Anti-Phosphotyrosine purified antibody (concentration in sample 2 μg/mL, GAM FITC, red-filled histogram) binds specifically to surface phosphotyrosines in EGF treated A431 cells (upper panel), but not to the untreated A431 cells (lower panel). (Phosphotyrosine Antikörper)

FACS

Phosphotyrosine pattern in HeLa cells as revealed by P-Tyr-01 antibody-Alexa Fluor ® 488 (green). (Phosphotyrosine Antikörper)

2 Abbildungen

(3 Referenzen)

Produktnummer ABIN94452

Datenblatt (PDF)

Target Alle Phosphotyrosine Produkte

Phosphotyrosine
Reaktivität
15

15

15

4
Diverse Spezies
Wirt
90

27

1
Maus
Passende Sekundärantikörper
Klonalität
100

18
Monoklonal
Konjugat
38

17

11

8

4

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser Phosphotyrosine Antikörper ist unkonjugiert
Applikation
82

62

40

37

33

22

21

17

13

9

8

7

7

6

3

2

2

2

1

1

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Spezifität

The antibody P-Tyr-01 detects tyrosine phosphorylation in activated cells.

Kreuzreaktivität (Details)

Broad

Aufreinigung

Purified by protein-A affinity chromatography.

Reinheit

> 95 % (by SDS-PAGE)

Immunogen

Phosphotyrosine conjugated to bovine serum albumin.

Klon

P-Tyr-01

Isotyp

IgG1
anti-Phosphotyrosine antibody
WB, IP, IF, IHC, ELISA, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Phosphotyrosine antibody
WB, ELISA Wirt: Maus Monoclonal unconjugated

anti-Phosphotyrosine antibody
Reaktivität: Diverse Spezies WB, IHC (p) Wirt: Maus Monoclonal 3F6 unconjugated

anti-Phosphotyrosine antibody
Reaktivität: Diverse Spezies WB, IHC (p) Wirt: Maus Monoclonal unconjugated

anti-Phosphotyrosine antibody
WB, IP, IF, IHC, ICC Wirt: Maus Monoclonal G104 unconjugated

anti-Phosphotyrosine antibody
WB, IP, IF, IHC, FACS, ISt, StM Wirt: Maus Monoclonal PY20 unconjugated

anti-Phosphotyrosine antibody (HRP)
WB, IP, IF, IHC, ICC Wirt: Maus Monoclonal G104 HRP

anti-Phosphotyrosine antibody (FITC)
WB, IP, IF, IHC, ICC Wirt: Maus Monoclonal G104 FITC

anti-Phosphotyrosine antibody (Biotin)
WB, IP, IF, IHC, ICC Wirt: Maus Monoclonal G104 Biotin

anti-Phosphotyrosine antibody (PE)
WB, IP, IF, IHC, ICC Wirt: Maus Monoclonal G104 PE

Applikationshinweise

Western blotting: Recommended dilution: 1-2 μg/mL. Flow cytometry: Recommended dilution: 2-5 μg/mL. Intracellular staining.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Konzentration

1 mg/mL

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung

Do not freeze.

Lagerung

4 °C

Informationen zur Lagerung

Store at 2-8°C. Do not freeze.
Biebl, Riedl, Buchner: "Hsp90 Co-chaperones Form Plastic Genetic Networks Adapted to Client Maturation." in: Cell reports, Vol. 32, Issue 8, pp. 108063, (2020) (PubMed).

Meraner, Horejsí, Wolpl, Fischer, Stingl, Maurer: "Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 4, pp. 2262-71, (2007) (PubMed).

Brdicka, Imrich, Angelisová, Brdicková, Horváth, Spicka, Hilgert, Lusková, Dráber, Novák, Engels, Wienands, Simeoni, Osterreicher, Aguado, Malissen, Schraven, Horejsí: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, (2002) (PubMed).
Target

Phosphotyrosine

Abstract

Phosphotyrosine Produkte

Substanzklasse

Amino Acid

Hintergrund

PY