SYK Antikörper (AA 5-360)

Produktnummer ABIN94475

Produktdetails anti-SYK Antikörper

Target: SYK (Spleen tyrosine Kinase (SYK))

Bindungsspezifität: AA 5-360

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SYK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Spezifität: The antibody SYK-01 reacts with protein tyrosine kinase p72Syk (Syk, an intracellular antigen), which is required for the transduction of signals through the B cell antigen receptor (BCR) and the high affinity IgE receptor (FcepsilonRI).

Kreuzreaktivität (Details): Mouse, Human, Rat, Other not tested

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Recombinant fragment (aa 5-360) of human Syk.

Klon: SYK-01

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blotting: Recommended dilution: 1-2 μg/mL.
Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/mL, positive tissue: tonsil B cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-SYK Antikörper (ABIN94475)

Hasan, Rahman, Sebo, Osicka: "Distinct Spatiotemporal Distribution of Bacterial Toxin-Produced Cellular cAMP Differentially Inhibits Opsonophagocytic Signaling." in: Toxins, Vol. 11, Issue 6, (2020) (PubMed).

Heneberg, Lebduska, Dráberová, Korb, Dráber: "Topography of plasma membrane microdomains and its consequences for mast cell signaling." in: European journal of immunology, Vol. 36, Issue 10, pp. 2795-806, (2006) (PubMed).

Hálová, Dráberová, Dráber: "A novel lipid raft-associated glycoprotein, TEC-21, activates rat basophilic leukemia cells independently of the type 1 Fc epsilon receptor." in: International immunology, Vol. 14, Issue 2, pp. 213-23, (2002) (PubMed).

Arudchandran, Brown, Peirce, Song, Zhang, Siraganian, Blank, Rivera: "The Src homology 2 domain of Vav is required for its compartmentation to the plasma membrane and activation of c-Jun NH(2)-terminal kinase 1." in: The Journal of experimental medicine, Vol. 191, Issue 1, pp. 47-60, (2000) (PubMed).

Details zu SYK

Target: SYK (Spleen tyrosine Kinase (SYK))

Andere Bezeichnung: Syk (SYK Produkte)

Synonyme: p72-Syk antikoerper, Sykb antikoerper, p72syk antikoerper, spleen associated tyrosine kinase antikoerper, spleen tyrosine kinase antikoerper, syk antikoerper, SYK antikoerper, Syk antikoerper

Hintergrund: Spleen associated tyrosine kinase,Syk is a cytoplasmic protein tyrosine kinase that translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.,p72Syk

Gen-ID: 6850

UniProt: P43405

Pathways: Fc-epsilon Rezeptor Signalübertragung, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Thromboxane A2 Receptor Signaling, BCR Signaling