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Vimentin Antikörper

VIM Reaktivität: Human WB, ELISA, FACS, IHC (p), ICC Wirt: Maus Monoclonal VI-RE-1 unconjugated
Produktnummer ABIN94494
  • Target Alle Vimentin (VIM) Antikörper anzeigen
    Vimentin (VIM)
    Reaktivität
    • 342
    • 135
    • 132
    • 61
    • 60
    • 50
    • 43
    • 38
    • 30
    • 22
    • 20
    • 14
    • 13
    • 8
    • 8
    • 6
    • 6
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Wirt
    • 195
    • 130
    • 11
    • 6
    • 6
    • 5
    • 2
    Maus
    Klonalität
    • 211
    • 143
    Monoklonal
    Konjugat
    • 204
    • 19
    • 14
    • 14
    • 10
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Dieser Vimentin Antikörper ist unkonjugiert
    Applikation
    • 286
    • 129
    • 107
    • 99
    • 97
    • 63
    • 45
    • 40
    • 39
    • 33
    • 23
    • 19
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
    Spezifität
    The antibody VI-RE/1 reacts with human vimentin, a 57 kDa intermediate filament intracellular protein expressed on a wide variety of mesenchymal and mesodermal cell types.
    Keine Kreuzreaktivität
    Maus, Schwein
    Kreuzreaktivität (Details)
    Human
    Aufreinigung
    Purified by protein-A affinity chromatography.
    Reinheit
    > 95 % (by SDS-PAGE)
    Immunogen
    Bacterially expressed full-length human vimentin
    Klon
    VI-RE-1
    Isotyp
    IgG1
    Top Product
    Discover our top product VIM Primärantikörper
  • Applikationshinweise
    ELISA: The antibody VI-RE/1 recognizes different epitope on human vimentin than the antibody VI-01 (IgM).
    Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining.
    Western blotting: Recommended dilution: 1-2 μg/mL.
    Immunocytochemistry: Recommended dilution: 5-10 μg/mL.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Do not freeze.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store at 2-8°C. Do not freeze.
  • Chen, Chang, Wu, Li, Yang, Chen, Hsu, Chen, Wu, Lee, Huang, Goan, Chou, Huang, Wu: "Molecular characterization of invasive subpopulations from an esophageal squamous cell carcinoma cell line." in: Anticancer research, Vol. 30, Issue 3, pp. 727-36, (2010) (PubMed).

  • Target
    Vimentin (VIM)
    Andere Bezeichnung
    Vimentin (VIM Produkte)
    Synonyme
    CTRCT30 antikoerper, cb28 antikoerper, vime antikoerper, vim antikoerper, vim1 antikoerper, vim2 antikoerper, VIM antikoerper, Vimentin antikoerper, vim4 antikoerper, vimentin antikoerper, vimentin L homeolog antikoerper, vimentin S homeolog antikoerper, VIM antikoerper, Vim antikoerper, vim antikoerper, vim.L antikoerper, vim.S antikoerper
    Hintergrund
    Vimentin,Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types - fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. In non-vascular smooth muscle cellsand striated muscle, vimentin is often replaced by desmin, however, during regeneration, vimentin is reexpressed. Cells of the lymfo-haemopoietic system (lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts. Vimentin is also found in mesoderm derived epithelia, e.g. kidney (Bowman capsule), endometrium and ovary (surface epithelium), in myoepithelial cells (breast, salivary and sweat glands), an in thyroid gland epithelium. In these cell types, as in mesothelial cells, vimentin is coexpressed with cytokeratin.Furthermore, vimentin is detected in many cells from the neural crest. Particularly melanocytes express abundant vimentin. In glial cells vimentin is coexpressed with Glial Fibrillary Acidic Protein (GFAP). Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive. Mesoderm derived carcinomas like renal cell carcinoma, adrenal cortical carcinoma and adenocarcinomas from endometrium and ovary usually express vimentin. Also thyroid carcinomas are vimentin positive. Any low differentiated carcinoma may express some vimentin. Vimentin is frequently included in the so-called primary panel (together with CD45, cytokeratin, and S-100 protein). Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is strongly suggestive of a mesenchymal tumour or malignant melanoma.
    Gen-ID
    7431
    UniProt
    P08670
    Pathways
    Caspase Kaskade in der Apoptose
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