ALD2 Antikörper

Produktnummer ABIN94896

Hersteller Produkt- Nr.: 200-4144-0100

Produktdetails

Target: ALD2

Reaktivität: Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: ELISA, Immunoprecipitation (IP), Western Blotting (WB)

Hersteller Produkt- Nr.: 200-4144-0100

Hersteller: Rockland

Verwendungszweck: Aldehyde Dehydrogenase Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Aldehyde Dehydrogenase [Yeast].

Produktmerkmale: Synonyms: rabbit anti-Aldehyde Dehydrogenase Antibody, Aldehyde dehydrogenase [NAD(P)+] 1, ALD2, ALD5, ALD

Aufreinigung: Anti-Aldehyde Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Aldehyde Dehydrogenase [Yeast]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Aldehyde Dehydrogenase has been tested by western blot. Assays should be optimized by the end user for specific conditions for reactivity.

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Antigendetails

Target: ALD2

Hintergrund: Background: The enzyme encoded by this gene belongs to the aldehyde dehydrogenase family of enzymes that catalyze the chemical transformation from acetaldehyde to acetic acid. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. The ALDH2 gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix, in contrast the ALDH1 gene codes for the cytosolic isoform. Anti-ALDEHYDE DEHYDROGENASE is ideal for researched interested in metabolism research.

Gen-ID: 855206

NCBI Accession: NP_013893

UniProt: P47771