Dieser Alkaline Phosphatase Antikörper ist unkonjugiert
Applikation
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Kreuzreaktivität (Details)
Cross reactivity against Alkaline Phosphatase from other tissues and species may occur but have not been specifically determined.
Reinheit
Anti-ALKALINE PHOSPHATASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine].
Endotoxin-Niveau
Low Endotoxin : No
Immunogen
Alkaline Phosphatase [Calf Intestine] Immunogen Type: Native Protein
ALP
Reaktivität: Human
ELISA, IEM
Wirt: Schaf
Polyclonal
unconjugated
Applikationshinweise
ELISA Dilution: 1:20.000 - 1:100.000 Immunohistochemistry Dilution: 1:1.000 - 1:5.000 Western Blot Dilution: 1:2.000 - 1:10.000
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Handhabung
Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Haltbarkeit
Expiration date is one (1) year from date of opening.
Jones, Brindley, Elder, Myers, Rajala, Dekaney, McNamee, Frey, Shroyer, Dempsey: "Cellular Plasticity of Defa4Cre-Expressing Paneth Cells in Response to Notch Activation and Intestinal Injury." in: Cellular and molecular gastroenterology and hepatology, Vol. 7, Issue 3, pp. 533-554, (2019) (PubMed).