D Amino Acid Oxidase Antikörper

Produktnummer ABIN94968

Hersteller Produkt- Nr.: 200-601-143-0100

Produktdetails anti-D Amino Acid Oxidase Antikörper

Target: D Amino Acid Oxidase (DAO) (D-Amino-Acid Oxidase (DAO))

Reaktivität: Schwein

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser D Amino Acid Oxidase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Hersteller Produkt- Nr.: 200-601-143-0100

Hersteller: Rockland

Verwendungszweck: D-Amino Acid Oxidase Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified D-Amino Acid Oxidase [Pig Kidney].

Produktmerkmale: Synonyms: sheep anti-D-Amino Acid Oxidase Antibody, D-amino-acid oxidase, DAMOX, DAAO, DAO

Aufreinigung: Anti-D-AMINO ACID OXIDASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: D-Amino Acid Oxidase [Pig Kidney]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: D-AMINO ACID OXIDASE antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of D-Amino Acid Oxidase [Pig Kidney] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) code #611-1302 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:60,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu D Amino Acid Oxidase

Target: D Amino Acid Oxidase (DAO) (D-Amino-Acid Oxidase (DAO))

Andere Bezeichnung: DAO (DAO Produkte)

Hintergrund: Background: D-Amino Acid Oxidase regulates the level of the neuromodulator D-serine in the brain. It has high activity towards D-DOPA and contributes to dopamine synthesis. It could act as a detoxifying agent which removes D-amino acids accumulated during aging. It acts on a variety of D-amino acids, with a preference for those having small hydrophobic side chains, followed by those bearing polar, aromatic, and basic groups. D-Amino Acid Oxidase does not act on acidic amino acids.

Gen-ID: 397134

NCBI Accession: NP_999231

UniProt: P00371