Angiopoietin 1 Antikörper

Produktnummer ABIN95109

Produktdetails anti-Angiopoietin 1 Antikörper

Target: Angiopoietin 1 (ANGPT1)

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Angiopoietin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktmerkmale: Concentration Definition: by Refractometry

Immunogen: This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide N-Q-R-R-N-P-E-N-G-G-R-R-Y-N-R-I-Q-H-G-Q corresponding to a region (aa 21-40) near the of mouse angiopoietin 1 protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.

Anwendungsinformationen

Applikationshinweise: Suitable for western blotting, Immunohistochemistry (IHC) and other antibody based assays.  A 1:500 dilution is recommended for western blotting.  The reaction of this antiserum directly with cell supernatants may result in high background due to reactivity of components in the serum.  This can be alleviated by first immunoprecipitating the antibody:antigen complex and then detecting the antigen. This method results in a very clean and strong signal.  Both Ang-1 and Ang-2 proteins have predicted molecular weights of approximately 57 kDa and appear on western blots close to their predicted molecular weights.  In some instances additional bands may be seen at approximately 75 kDa which represent highly glycosylated forms of the protein that migrate at a higher apparent molecular weight.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 85 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Referenzen für anti-Angiopoietin 1 Antikörper (ABIN95109)

Egginton, Hussain, Hall-Jones, Chaudhry, Syeda, Glen: "Shear stress-induced angiogenesis in mouse muscle is independent of the vasodilator mechanism and quickly reversible." in: Acta physiologica (Oxford, England), Vol. 218, Issue 3, pp. 153-166, (2017) (PubMed).

Dallabrida, Ismail, Pravda, Parodi, Dickie, Durand, Lai, Cassiola, Rogers, Rupnick: "Integrin binding angiopoietin-1 monomers reduce cardiac hypertrophy." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 22, Issue 8, pp. 3010-23, (2008) (PubMed).

Câmpean, Karpe, Haas, Atalla, Peters, Rupprecht, Liebner, Acker, Plate, Amann: "Angiopoietin 1 and 2 gene and protein expression is differentially regulated in acute anti-Thy1.1 glomerulonephritis." in: American journal of physiology. Renal physiology, Vol. 294, Issue 5, pp. F1174-84, (2008) (PubMed).

Details zu Angiopoietin 1

Target: Angiopoietin 1 (ANGPT1)

Andere Bezeichnung: Angiopoietin 1 (ANGPT1 Produkte)

Synonyme: AGP1 antikoerper, AGPT antikoerper, ANG1 antikoerper, 1110046O21Rik antikoerper, Ang-1 antikoerper, Ang1 antikoerper, Agpt antikoerper, Agpt1 antikoerper, ang1 antikoerper, wu:fc76g06 antikoerper, ANG-1 antikoerper, angpt1 antikoerper, MGC80788 antikoerper, angiopoietin 1 antikoerper, angiopoietin 1 S homeolog antikoerper, ANGPT1 antikoerper, Angpt1 antikoerper, angpt1 antikoerper, angpt1.S antikoerper

Hintergrund: Anti Antiopoietin-1 Antibody recognizes Angiopoietin-1 (Ang-1) that has importance in the development of the endothelium by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has a potential fibrinogen-like domain at the carboxyl terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, Ang-1 is also expressed in the heart and liver.
Synonyms: AGP 1 antibody, AGP1 antibody, AGPT antibody, ANG 1 antibody, ANG1 antibody, Angiopoietin1 antibody, ANGPT 1 antibody, ANGPT1 antibody, KIAA0003 antibody

Gen-ID: 11600, 46048213

NCBI Accession: NP_031452

UniProt: O08538

Pathways: RTK Signalweg, Glycosaminoglycan Metabolic Process