This monoclonal antibody detects an epitope on the N terminus (aa 1-15) of the human C5a receptor (also known as CD88) found on human myeloid cells including granulocytes and monocytes/macrophages. It does not bind to tubular epithelial cells in situ under normal or inflammatory conditions. C5aR binds to C5a, a 74 aa peptide cleaved from complement protein C5. C5a receptor has seven transmembrane segments and is a member of the rhodopsin subfamily of G protein coupled receptors.
Aufreinigung
Affinity chromatography on Protein G
Immunogen
RBL-2H3 (rat basophilic leukemia) transfected cells expressing human C5aR on the cell surface Donor: Lou/c rat spleen cells
C5AR1
Reaktivität: Human
WB, PLA
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Optimal working dilution should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
0,1 mg/mL
Buffer
PBS containing 0.02 % sodium azide (NaN3) as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.