Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Hersteller Produkt- Nr.
200-4197-0100
Hersteller
Rockland
Kreuzreaktivität (Details)
No cross reactivity occurs against Streptavidin.
Reinheit
Anti-AVIDIN is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Avidin [Hen Egg White].
Endotoxin-Niveau
Low Endotoxin : No
Immunogen
Avidin (Hen Egg White) Immunogen Type: Native Protein
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. ELISA Dilution: 1:2.000 - 1:20.000 Immunohistochemistry Dilution: 1:1.000 - 1:5.000 Western Blot Dilution: 1:1.000 - 1:10.000
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Handhabung
Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Haltbarkeit
Expiration date is one (1) year from date of opening.