FITC Antikörper

Produktnummer ABIN958555

Produktdetails anti-FITC Antikörper

Target: FITC (Fluorescein Isothiocyanate (FITC))

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FITC Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB)

Spezifität: FITC. The reactivity of the antiserum is directed to the FITC molecule as tested in direct binding enzyme immunoassay, ELISA and immunoblotting. Cross-reactivity This antiserum has not been tested for cross-reactivity with other fluorescent compounds.

Aufreinigung: Purified IgG

Immunogen: FITC

Type of Immunogen: Purified protein

Klon: NI 239

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, WB

Usage: This product is intended for use to enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to FITC. The phenomenon of a weak reaction of a monoclonal antibody is e. g. well known in different analysis of vital peripheral blood mononuclear cells in suspensions by the expression of surface markers. A similar situation exists in solid phase assay systems (ELISA, blotting, DIBA) when used for the identification and /or quantitative determination of minute amounts of soluble specific antigens or antibodies. The sensitivity of the FITC hapten-anti-hapten system makes it a valuable alternative to the biotinavidin system. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1000 upwards. These data should be interpreted as general recommendations only.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile distilled water 0.5 ml

Konzentration: Lot specific

Buffer: Lyophilized from PBS, pH 7.2

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.

Details zu FITC

Target: FITC (Fluorescein Isothiocyanate (FITC))

Andere Bezeichnung: FITC (FITC Produkte)

Substanzklasse: Chemical

Hintergrund: Name/Gene ID: FITC

Synonyms: FITC