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Collagen IV Antikörper

COL4 Reaktivität: Human ELISA, IHC, IHC (fro), IHC (p), IF Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN961130
  • Target Alle Collagen IV (COL4) Antikörper anzeigen
    Collagen IV (COL4) (Collagen, Type IV (COL4))
    Reaktivität
    • 97
    • 49
    • 41
    • 23
    • 16
    • 11
    • 5
    • 2
    Human
    Wirt
    • 62
    • 30
    • 12
    Kaninchen
    Klonalität
    • 73
    • 31
    Polyklonal
    Konjugat
    • 48
    • 10
    • 7
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Collagen IV Antikörper ist unkonjugiert
    Applikation
    • 65
    • 47
    • 31
    • 30
    • 30
    • 26
    • 26
    • 25
    • 15
    • 9
    • 9
    • 8
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
    Marke
    IHC-plus™
    Spezifität
    Human Type IV collagen. Cross-reactivity: Human collagen type I: 1 % Human collagen type II: <0.5 %, Human collagen type III: <0.5 %, Human collagen type IV: 100 %, Human collagen type V: 7 %, Human fibronectin: <0.5 %, Human albumin: <0.5 %, Human immunoglobulin <0.5 %.
    Aufreinigung
    Affinity purified
    Immunogen
    Human placental collagen type IV.

    Type of Immunogen: Purified protein
    Isotyp
    IgG
    Top Product
    Discover our top product COL4 Primärantikörper
  • Applikationshinweise
    Approved: ELISA (1:3000), IF, IHC, IHC-Fr, IHC-P (1:100)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3654 was determined to be 1:100. Suitable for use in ELISA, Indirect immunofluorescence, indirect immunohistochemistry and Dot Blotting (not suitable for Western Blot). ELISA: 1:3000. Indirect immunofluorescent visualization of collagen type IV on cryostat sections of human tissue: 1:20-1:40. Indirect Immunohistochemistry: 1:20-1:40. fresh frozen or acetone fixed specimens. Dot Blotting: 1:300. not recommended for Western Blots.

    Not recommended for: WB
    Kommentare

    Target Species of Antibody: Human

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    PBS, mannitol, dextran, no preservatives.
    Konservierungsmittel
    Without preservative
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    4°C or -20°C, Avoid freeze-thaw cycles.
  • Target
    Collagen IV (COL4) (Collagen, Type IV (COL4))
    Andere Bezeichnung
    Collagen IV (COL4 Produkte)
    Synonyme
    collagen, type IV antikoerper, Let-2 antikoerper
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