Collagen IV Antikörper
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- Target Alle Collagen IV (COL4) Antikörper anzeigen
- Collagen IV (COL4) (Collagen, Type IV (COL4))
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser Collagen IV Antikörper ist unkonjugiert
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Applikation
- ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
- Marke
- IHC-plus™
- Spezifität
- Human Type IV collagen. Cross-reactivity: Human collagen type I: 1 % Human collagen type II: <0.5 %, Human collagen type III: <0.5 %, Human collagen type IV: 100 %, Human collagen type V: 7 %, Human fibronectin: <0.5 %, Human albumin: <0.5 %, Human immunoglobulin <0.5 %.
- Aufreinigung
- Affinity purified
- Immunogen
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Human placental collagen type IV.
Type of Immunogen: Purified protein - Isotyp
- IgG
- Top Product
- Discover our top product COL4 Primärantikörper
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- Applikationshinweise
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Approved: ELISA (1:3000), IF, IHC, IHC-Fr, IHC-P (1:100)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3654 was determined to be 1:100. Suitable for use in ELISA, Indirect immunofluorescence, indirect immunohistochemistry and Dot Blotting (not suitable for Western Blot). ELISA: 1:3000. Indirect immunofluorescent visualization of collagen type IV on cryostat sections of human tissue: 1:20-1:40. Indirect Immunohistochemistry: 1:20-1:40. fresh frozen or acetone fixed specimens. Dot Blotting: 1:300. not recommended for Western Blots.
Not recommended for: WB - Kommentare
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Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- Lot specific
- Buffer
- PBS, mannitol, dextran, no preservatives.
- Konservierungsmittel
- Without preservative
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- Target
- Collagen IV (COL4) (Collagen, Type IV (COL4))
- Andere Bezeichnung
- Collagen IV (COL4 Produkte)
- Synonyme
- collagen, type IV antikoerper, Let-2 antikoerper
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